Recording transcriptional histories using Record-seq

• Published in: Nature protocols Vol. 15; no. 2; pp. 513 - 539
• Main Authors: Tanna, Tanmay, Schmidt, Florian, Cherepkova, Mariia Y., Okoniewski, Michal, Platt, Randall J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.02.2020; Nature Publishing Group
• Subjects: 631/1647/2017; 631/1647/48; 631/1647/514/1949; 631/337/2019; 631/337/4041/3196; Amplification; Analytical Chemistry; Arrays; Biological Techniques; Biomedical and Life Sciences; Clustered Regularly Interspaced Short Palindromic Repeats - genetics; Computational Biology/Bioinformatics; CRISPR; Data analysis; Design of experiments; Escherichia coli - genetics; Experimental design; Gene sequencing; Genetic transcription; Genomes; History; Life Sciences; Microarrays; Organic Chemistry; Protocol; Recording; Ribonucleic acid; RNA; Sequence Analysis, DNA - methods; Technology application; Transcription; Transcription, Genetic; Workflow; Switzerland

It is difficult to elucidate the transcriptional history of a cell using current experimental approaches, as they are destructive in nature and therefore describe only a moment in time. To overcome these limitations, we recently established Record-seq, a technology that enables transcriptional recor...