Determination of Drug Efflux Pump Efficiency in Drug-Resistant Bacteria Using MALDI-TOF MS

• Published in: Antibiotics (Basel) Vol. 9; no. 10; p. 639
• Main Authors: Lu, Wen-Jung, Lin, Hsuan-Ju, Hsu, Pang-Hung, Lin, Hong-Ting Victor
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.10.2020; MDPI
• Subjects: AcrB; Antibiotic resistance; Antibiotics; Bacteria; Drug resistance; Dyes; E coli; Efflux; efflux activity; Fluorescence; MALDI-TOF MS; Methods; Microbial drug resistance; Minimum inhibitory concentration; multidrug transporter; Observations; Proteins; Rate constants; Substrates; Time-of-flight mass spectrometry; Tuberculosis; Taiwan

Multidrug efflux pumps play an essential role in antibiotic resistance. The conventional methods, including minimum inhibitory concentration and fluorescent assays, to monitor transporter efflux activity might have some drawbacks, such as indirect evidence or interference from color molecules. In th...