Genetic and biological characterisation of Zika virus isolates from different Brazilian regions

Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal...

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Published inMemórias do Instituto Oswaldo Cruz Vol. 114; p. e190150
Main Authors Strottmann, Daisy Maria, Zanluca, Camila, Mosimann, Ana Luiza Pamplona, Koishi, Andrea C, Auwerter, Nathalia Cavalheiro, Faoro, Helisson, Cataneo, Allan Henrique Depieri, Kuczera, Diogo, Wowk, Pryscilla Fanini, Bordignon, Juliano, Duarte Dos Santos, Claudia Nunes
Format Journal Article
LanguageEnglish
Portuguese
Published Brazil Instituto Oswaldo Cruz, Ministério da Saúde 01.01.2019
Fundação Oswaldo Cruz (FIOCRUZ)
Subjects
biological characterisation
Flavivirus
molecular markers
Original
PARASITOLOGY
TROPICAL MEDICINE
Zika virus
Zika virus
biological characterisation
Flavivirus
molecular markers
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Summary:Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 Brazilian epidemics. The ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. Eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the well-established ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.
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DMS, CZ, ACK, ALPM, PFW, JB and CNDS conceived and designed the study; DMS, CZ, ACK, ALPM, AHDC, DK, PFW, JB and CNDS wrote the manuscript; CZ performed the virus isolation procedures; NCA amplified the ZIKV genome, and HF performed the sequencing; DMS, CZ, ACK, AHDC, DK and PFW carried out the experiments; DMS and ALPM analysed and compared the ZIKV genomes; ALPM performed the phylogenetic analyses. All authors read and approved the final manuscript. The authors declare no conflicts of interest.
ISSN:0074-0276
1678-8060
1678-8060
DOI:10.1590/0074-02760190150