Effects of age on parathyroid hormone signaling in human marrow stromal cells

• Published in: Aging cell Vol. 10; no. 5; pp. 780 - 788
• Main Authors: Zhou, Shuanhu, Bueno, Ericka M., Kim, Sung Won, Amato, Ilaria, Shen, Longxiang, Hahne, Jochen, Bleiberg, Ilan, Morley, Paul, Glowacki, Julie
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.10.2011; John Wiley & Sons, Inc
• Subjects: Adult; age; Age Factors; Aged; Aging - drug effects; Aging - physiology; Alkaline Phosphatase - metabolism; beta Catenin - metabolism; Blotting, Western; Bone marrow; Bone Marrow Cells - cytology; Cell Differentiation; Cell Proliferation; Cells, Cultured; CREB; Cyclic AMP Response Element-Binding Protein - metabolism; Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors; Dexamethasone - pharmacology; differentiation; Electroporation; Female; human marrow stromal cells; Humans; Isoquinolines - pharmacology; Kinases; Male; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; Mesenchymal Stem Cells - physiology; Middle Aged; osteoblast; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - physiology; parathyroid hormone; Parathyroid Hormone - pharmacology; proliferation; Receptor, Parathyroid Hormone, Type 1 - genetics; Receptor, Parathyroid Hormone, Type 1 - metabolism; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; Signal Transduction; signaling; Stromal Cells - cytology; Sulfonamides - pharmacology; Transfection; β‐catenin

Summary Human bone marrow stromal cells (hMSCs) have the potential to differentiate into osteoblasts; there are age‐related decreases in their proliferation and differentiation to osteoblasts. Parathyroid hormone (PTH), when applied intermittently in vivo, has osteoanabolic effects in a variety of s...