A fluorescence study of the binding of Hoechst 33258 and DAPI to halogenated DNAs

• Published in: Photochemistry and photobiology Vol. 51; no. 1; p. 77
• Main Authors: Härd, T, Fan, P, Kearns, D R
• Format: Journal Article
• Language: English
• Published: United States 01.01.1990
• Subjects: Benzimidazoles - metabolism; Bisbenzimidazole - metabolism; Bromodeoxycytidine; DNA - metabolism; Fluorescence; Fluorescent Dyes; Halogens - metabolism; Idoxuridine; Indoles - metabolism; Photochemistry; Polydeoxyribonucleotides - metabolism; Spectrometry, Fluorescence

We have studied the time-resolved and the steady-state fluorescence of the DNA groove binders 4',6-diamidino-2-phenylindole (DAPI) and Hoechst 33258 with the double stranded DNAs poly(dA-dU) and poly(dI-dC) and their halogenated analogs, poly(dA-I5dU) and poly(dI-Br5dC). These studies were prom...