Expression, characterization, and crystallization of a member of the novel phospholipase D family of phosphodiesterases

• Published in: Protein science Vol. 6; no. 12; pp. 2655 - 2658
• Main Authors: Zhao, Yi, Stuckey, Jeanne A., Lohse, Daniel L., Dixon, Jack E.
• Format: Journal Article
• Language: English
• Published: Bristol Cold Spring Harbor Laboratory Press 01.12.1997
• Subjects: Amino Acid Sequence; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Cations, Divalent; Crystallization; Crystallography, X-Ray; DNA - metabolism; Edetic Acid - pharmacology; Endonucleases; Escherichia coli - genetics; Gene Expression; Micrococcal Nuclease; Molecular Sequence Data; nuclease; phosphodiester; phospholipase D; Plasmids - genetics; Recombinant Proteins; Salmonella typhimurium - enzymology; Salmonella typhimurium - genetics

A family of phospholipase D (PLD) proteins has recently been identified (Koonin, 1996; Ponting & Kerr, 1996) based upon amino acid sequence identity. This family includes human and plant PLDs, proteins encoded by open reading frames in pathogenic viruses and bacteria, as well as an endonuclease....