Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile pseudomonas aeruginosa

A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained in the presence of PEG 6000 at pH 7 and pH 8. They belong to the space group P212121. A complete data set to 2.1 Å resolution has been measured. The st...

Full description

Saved in:
Bibliographic Details
Published inProtein science Vol. 6; no. 11; pp. 2462 - 2464
Main Authors Villeret, Vincent, Beeumen, Jozef Van, Chessa, Jean‐Pierre, Gerday, Charles
Format Journal Article
LanguageEnglish
Published Bristol Cold Spring Harbor Laboratory Press 01.11.1997
Subjects
Antarctic Regions
Bacterial Proteins - chemistry
Calcium
Cold Temperature
Crystallography, X-Ray
Metalloendopeptidases - chemistry
Models, Molecular
protease
Pseudomonas aeruginosa - enzymology
psychrophilic enzymes
Sequence Homology, Amino Acid
Serine Endopeptidases
X‐ray crystallography
Zinc
Antarctic Regions
Online AccessGet full text

Cover

Abstract A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained in the presence of PEG 6000 at pH 7 and pH 8. They belong to the space group P212121. A complete data set to 2.1 Å resolution has been measured. The structure has been determined by the molecular replacement method using the coordinates of the mesophilic alkaline protease as a model. The molecular replacement solution displays a correlation coefficient of 0.39 and an R‐factor of 0.48. Subsequent inspection of the electron density map in the active site region has confirmed the correctness of the solution. Model building and structure refinement will be initiated when the protease sequence becomes fully available. This is the second report, following one on an a‐amylase, of the preliminary crystallographic characterization of a psychrophilic enzyme.
AbstractList A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained in the presence of PEG 6000 at pH 7 and pH 8. They belong to the space group P2(1)2(1)2(1). A complete data set to 2.1 A resolution has been measured. The structure has been determined by the molecular replacement method using the coordinates of the mesophilic alkaline protease as a model. The molecular replacement solution displays a correlation coefficient of 0.39 and an R-factor of 0.48. Subsequent inspection of the electron density map in the active site region has confirmed the correctness of the solution. Model building and structure refinement will be initiated when the protease sequence becomes fully available. This is the second report, following one on an alpha-amylase, of the preliminary crystallographic characterization of a psychrophilic enzyme.
A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained in the presence of PEG 6000 at pH 7 and pH 8. They belong to the space group P212121. A complete data set to 2.1 Å resolution has been measured. The structure has been determined by the molecular replacement method using the coordinates of the mesophilic alkaline protease as a model. The molecular replacement solution displays a correlation coefficient of 0.39 and an R‐factor of 0.48. Subsequent inspection of the electron density map in the active site region has confirmed the correctness of the solution. Model building and structure refinement will be initiated when the protease sequence becomes fully available. This is the second report, following one on an a‐amylase, of the preliminary crystallographic characterization of a psychrophilic enzyme.
Abstract A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained in the presence of PEG 6000 at pH 7 and pH 8. They belong to the space group P2 1 2 1 2 1 . A complete data set to 2.1 Å resolution has been measured. The structure has been determined by the molecular replacement method using the coordinates of the mesophilic alkaline protease as a model. The molecular replacement solution displays a correlation coefficient of 0.39 and an R ‐factor of 0.48. Subsequent inspection of the electron density map in the active site region has confirmed the correctness of the solution. Model building and structure refinement will be initiated when the protease sequence becomes fully available. This is the second report, following one on an a‐amylase, of the preliminary crystallographic characterization of a psychrophilic enzyme.
Author Beeumen, Jozef Van
Chessa, Jean‐Pierre
Gerday, Charles
Villeret, Vincent
AuthorAffiliation Laboratorium voor Eiwitbiochemie en Eiwitengineering, Universiteit Gent, Belgium
AuthorAffiliation_xml – name: Laboratorium voor Eiwitbiochemie en Eiwitengineering, Universiteit Gent, Belgium
Author_xml – sequence: 1
  givenname: Vincent
  surname: Villeret
  fullname: Villeret, Vincent
– sequence: 2
  givenname: Jozef Van
  surname: Beeumen
  fullname: Beeumen, Jozef Van
  email: jozef.vanbeeumen@rug.ac.be
– sequence: 3
  givenname: Jean‐Pierre
  surname: Chessa
  fullname: Chessa, Jean‐Pierre
– sequence: 4
  givenname: Charles
  surname: Gerday
  fullname: Gerday, Charles
BackLink https://www.ncbi.nlm.nih.gov/pubmed/9385650$$D View this record in MEDLINE/PubMed
BookMark eNqFkc1v1DAQxS1UVLaFKzckn7hl8cSJE1-QqoovqVIr1AM3y3EmXYNjB9sBrfjncdlVKSdOo9Hv-c143hk58cEjIS-BbYGx-s0Sw7ZtBWMCoIYnZAONkFUvxZcTsmFSQNVz0T8jZyl9ZYw1UPNTcip534qWbcivm4jOztbruKcm7lPWjqYcV5PXiHTEjPGeZhs8DRPNO6TafdPOeqRldkadkE4xzH_Qhc86mmwNXdLe7GJYdtYVYcJ1DHPwOlGNcb2zPiT9nDydtEv44ljPye37d7eXH6ur6w-fLi-uKtMygIprBsPYt51o5DQiMJD12AFiN7QgegE1cpTDWEs9MdPoYRoElmOgmcZBa35O3h5sl3WYcTToc9ROLdHO5dMqaKv-Jd7u1F34oWpoeCt5MXh9NIjh-4opq9kmg85pj2FNqpMNZ0J2Rbg9CE0MKUWcHoYAU_dplT6ov2mVB68er_YgP8ZTuDzwn-WK-_-4qZvP14-8fwNydak5
CitedBy_id crossref_primary_10_1093_femsle_fny062
crossref_primary_10_1016_S1389_1723_99_89021_5
crossref_primary_10_1074_jbc_M104432200
crossref_primary_10_1099_mic_0_025114_0
crossref_primary_10_1111_j_1574_6968_2000_tb09377_x
crossref_primary_10_1046_j_1432_1327_1999_00205_x
crossref_primary_10_1098_rstb_2002_1105
crossref_primary_10_1093_protein_13_10_711
crossref_primary_10_1134_S0003683812040138
crossref_primary_10_1002_prot_10264
crossref_primary_10_1016_S0167_4838_00_00018_2
crossref_primary_10_3109_10408410903485525
crossref_primary_10_1016_j_jtbi_2006_12_008
crossref_primary_10_1002_jobm_201000385
crossref_primary_10_1016_S0294_3506_99_80123_9
crossref_primary_10_1007_s12010_010_8927_y
crossref_primary_10_1046_j_1432_1327_2001_02353_x
crossref_primary_10_1016_S1381_1177_00_00200_9
crossref_primary_10_1078_0723_2020_00006
crossref_primary_10_1128_JB_185_14_4195_4203_2003
crossref_primary_10_3390_biology2020755
crossref_primary_10_3389_fmicb_2021_660134
crossref_primary_10_1006_bbrc_1999_0324
crossref_primary_10_1002_prot_10084
crossref_primary_10_1016_S1381_1177_03_00103_6
crossref_primary_10_1016_S0167_4838_00_00237_5
crossref_primary_10_1016_j_polar_2017_02_002
crossref_primary_10_1016_S0167_7799_99_01413_4
crossref_primary_10_1016_S0167_4838_00_00101_1
Cites_doi 10.1006/jmbi.1993.1098
10.1107/S0021889891004430
10.1002/j.1460-2075.1993.tb06009.x
10.1038/358164a0
10.1515/9781400855414.355
10.1016/S0021-9258(18)42754-8
10.1107/S0907444994003112
10.1016/0926-6569(63)90114-7
10.1002/pro.5560051021
10.1111/j.1574-6976.1996.tb00236.x
10.1016/0022-2836(68)90205-2
10.1007/978-3-642-78598-6_6
10.1016/B978-0-08-027377-8.50028-2
ContentType Journal Article
Copyright Copyright © 1997 The Protein Society
Copyright_xml – notice: Copyright © 1997 The Protein Society
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
5PM
DOI 10.1002/pro.5560061121
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList MEDLINE - Academic
MEDLINE


CrossRef
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Anatomy & Physiology
Chemistry
EISSN 1469-896X
EndPage 2464
ExternalDocumentID 10_1002_pro_5560061121
9385650
PRO5560061121
Genre miscellaneous
Research Support, Non-U.S. Gov't
Journal Article
Comparative Study
GeographicLocations Antarctic Regions
GeographicLocations_xml – name: Antarctic Regions
GroupedDBID ---
.GJ
05W
0R~
123
1L6
1OC
24P
29P
2WC
31~
33P
3SF
3WU
4.4
52U
53G
5RE
6TJ
8-0
8-1
8UM
A00
A8Z
AAESR
AAEVG
AAHHS
AAIHA
AANLZ
AAONW
AASGY
AAXRX
AAZKR
ABCUV
ABGDZ
ABLJU
ACAHQ
ACCFJ
ACCZN
ACFBH
ACGFO
ACGFS
ACIWK
ACPOU
ACPRK
ACQPF
ACXBN
ACXQS
ADBBV
ADEOM
ADIZJ
ADKYN
ADMGS
ADOZA
ADXAS
ADZMN
AEEZP
AEIGN
AEIMD
AENEX
AEQDE
AEUQT
AEUYR
AFBPY
AFFNX
AFFPM
AFGKR
AFPWT
AFRAH
AFZJQ
AHBTC
AHMBA
AIAGR
AITYG
AIURR
AIWBW
AJBDE
AJXKR
ALMA_UNASSIGNED_HOLDINGS
ALUQN
AMBMR
AMYDB
AOIJS
ATUGU
AUFTA
AZVAB
BFHJK
BHBCM
BMNLL
BMXJE
BNHUX
BOGZA
BRXPI
C1A
C45
CAG
COF
CS3
DCZOG
DIK
DRFUL
DRSTM
DU5
E3Z
EBD
EBS
EJD
EMOBN
ESTFP
F5P
G-S
GODZA
GX1
HGLYW
HH5
HYE
HZ~
IH2
LATKE
LEEKS
LITHE
LOXES
LUTES
LYRES
MEWTI
MRFUL
MRSTM
MSFUL
MSSTM
MXFUL
MXSTM
MY~
NNB
O66
O9-
OIG
OK1
OVD
P2P
P2W
P4E
PQQKQ
QRW
RCA
RIG
ROL
RPM
RWI
SJN
SUPJJ
SV3
TEORI
TR2
WBKPD
WIH
WIK
WIN
WNSPC
WOHZO
WOQ
WXSBR
WYISQ
WYJ
XV2
Y6R
YKV
ZGI
ZXP
ZZTAW
~02
~S-
ACXME
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
5PM
ID FETCH-LOGICAL-c5011-3a01bd857649fde10192d71ee7b5168612e3e9bd29af0c4abfb6e112ecfdbaa3
IEDL.DBID RPM
ISSN 0961-8368
IngestDate Tue Sep 17 21:12:19 EDT 2024
Sat Aug 17 00:35:42 EDT 2024
Fri Aug 23 01:54:56 EDT 2024
Thu May 23 23:01:01 EDT 2024
Sat Aug 24 00:43:02 EDT 2024
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 11
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c5011-3a01bd857649fde10192d71ee7b5168612e3e9bd29af0c4abfb6e112ecfdbaa3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
OpenAccessLink https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/pro.5560061121
PMID 9385650
PQID 79430697
PQPubID 23479
PageCount 3
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_2143593
proquest_miscellaneous_79430697
crossref_primary_10_1002_pro_5560061121
pubmed_primary_9385650
wiley_primary_10_1002_pro_5560061121_PRO5560061121
PublicationCentury 1900
PublicationDate November 1997
PublicationDateYYYYMMDD 1997-11-01
PublicationDate_xml – month: 11
  year: 1997
  text: November 1997
PublicationDecade 1990
PublicationPlace Bristol
PublicationPlace_xml – name: Bristol
– name: United States
PublicationTitle Protein science
PublicationTitleAlternate Protein Sci
PublicationYear 1997
Publisher Cold Spring Harbor Laboratory Press
Publisher_xml – name: Cold Spring Harbor Laboratory Press
References 1993; 12
1996; 18
1993; 229
1991; 24
1993; 50
1963; 73
1992; 267
1992; 358
1984
1995
1993
1981
1996; 5
1994; 50
1968; 33
1994; 20
8851694 - J Endocrinol Invest. 1996 Jan;19(1):59-70
8253063 - EMBO J. 1993 Sep;12(9):3357-64
5700707 - J Mol Biol. 1968 Apr 28;33(2):491-7
1319561 - Nature. 1992 Jul 9;358(6382):164-7
8897615 - Protein Sci. 1996 Oct;5(10):2128-9
8445658 - J Mol Biol. 1993 Feb 20;229(4):945-68
Navaza J. (e_1_2_1_16_1) 1993; 50
CCP4 (e_1_2_1_6_1) 1994; 50
e_1_2_1_7_1
e_1_2_1_5_1
e_1_2_1_3_1
e_1_2_1_12_1
e_1_2_1_4_1
e_1_2_1_13_1
e_1_2_1_10_1
e_1_2_1_2_1
e_1_2_1_11_1
e_1_2_1_17_1
e_1_2_1_15_1
e_1_2_1_9_1
Morihara K. (e_1_2_1_14_1) 1963; 73
Feller G (e_1_2_1_8_1) 1992; 267
References_xml – start-page: 355
  year: 1984
  end-page: 449
– volume: 24
  start-page: 409
  year: 1991
  end-page: 411
  article-title: Sparse matrix sampling: A screening method for crystallization of proteins
  publication-title: J Appl Crystallogr
– volume: 12
  start-page: 3357
  year: 1993
  end-page: 3364
  article-title: Three‐dimensional structure of the alkaline protease of : A two‐domain protein with a calcium binding parallel beta roll motif
  publication-title: EMBO J
– volume: 50
  start-page: 760
  year: 1994
  end-page: 763
  article-title: Collaborative Computing Project Number 4. The CCP4 suite: Programs for protein crystallography
  publication-title: Acta Crystallogr D
– volume: 18
  start-page: 189
  year: 1996
  end-page: 202
  article-title: Enzymes from psychrophilic organisms
  publication-title: FEMS Microbiol Rev
– start-page: 231
  year: 1981
  end-page: 244
– volume: 229
  start-page: 945
  year: 1993
  end-page: 968
  article-title: Refined 1.8 Å X‐ray crystal structure of astacin, a zine‐endopeptidase from the crayfish L. Structure determination, refinement, molecular structure and comparison with thermolysin
  publication-title: J Mol Biol
– year: 1995
– volume: 20
  start-page: 269
  year: 1994
  end-page: 295
  article-title: Molecular adaptations of enzymes from thermophilic and psychrophilic organisms
  publication-title: Adv Comp Environm Physiol
– volume: 33
  start-page: 491
  year: 1968
  end-page: 497
  article-title: Solvent content of protein crystals
  publication-title: J Mol Biol
– volume: 358
  start-page: 164
  year: 1992
  end-page: 167
  article-title: Structure of astacin and implications for activation of astacins and zinc‐ligation of collagenases
  publication-title: Nature
– start-page: 56
  year: 1993
  end-page: 62
– volume: 5
  start-page: 2128
  year: 1996
  end-page: 2129
  article-title: Crystallization and preliminary X‐ray diffraction studies of α‐amylase from the Antarctic psychrophile A23
  publication-title: Protein Sci
– volume: 267
  start-page: 5217
  year: 1992
  end-page: 5521
  article-title: Purification, characterization and nucleotide sequence of the thermolabile α‐amylase from the Antarctic psychrotroph A 23
  publication-title: J Biol Chem
– volume: 73
  start-page: 113
  year: 1963
  end-page: 124
  article-title: proteinase. Purification and general properties
  publication-title: Biochim Biophys Acta
– volume: 50
  year: 1993
  article-title: AMoRe: An automated package for molecular replacement
  publication-title: Acta Crystallogr A
– ident: e_1_2_1_10_1
  doi: 10.1006/jmbi.1993.1098
– ident: e_1_2_1_12_1
  doi: 10.1107/S0021889891004430
– ident: e_1_2_1_4_1
  doi: 10.1002/j.1460-2075.1993.tb06009.x
– volume: 50
  year: 1993
  ident: e_1_2_1_16_1
  article-title: AMoRe: An automated package for molecular replacement
  publication-title: Acta Crystallogr A
  contributor:
    fullname: Navaza J.
– ident: e_1_2_1_5_1
  doi: 10.1038/358164a0
– ident: e_1_2_1_11_1
  doi: 10.1515/9781400855414.355
– ident: e_1_2_1_17_1
– volume: 267
  start-page: 5217
  year: 1992
  ident: e_1_2_1_8_1
  article-title: Purification, characterization and nucleotide sequence of the thermolabile α‐amylase from the Antarctic psychrotroph Alteromonas haloplanctis A 23
  publication-title: J Biol Chem
  doi: 10.1016/S0021-9258(18)42754-8
  contributor:
    fullname: Feller G
– volume: 50
  start-page: 760
  year: 1994
  ident: e_1_2_1_6_1
  article-title: Collaborative Computing Project Number 4. The CCP4 suite: Programs for protein crystallography
  publication-title: Acta Crystallogr D
  doi: 10.1107/S0907444994003112
  contributor:
    fullname: CCP4
– volume: 73
  start-page: 113
  year: 1963
  ident: e_1_2_1_14_1
  article-title: Pseudomonas aeruginosa proteinase. Purification and general properties
  publication-title: Biochim Biophys Acta
  doi: 10.1016/0926-6569(63)90114-7
  contributor:
    fullname: Morihara K.
– ident: e_1_2_1_2_1
  doi: 10.1002/pro.5560051021
– ident: e_1_2_1_9_1
  doi: 10.1111/j.1574-6976.1996.tb00236.x
– ident: e_1_2_1_13_1
  doi: 10.1016/0022-2836(68)90205-2
– ident: e_1_2_1_3_1
  doi: 10.1007/978-3-642-78598-6_6
– ident: e_1_2_1_7_1
  doi: 10.1016/B978-0-08-027377-8.50028-2
– ident: e_1_2_1_15_1
SSID ssj0004123
Score 1.7594286
Snippet A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained in the...
Abstract A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained...
SourceID pubmedcentral
proquest
crossref
pubmed
wiley
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 2462
SubjectTerms Antarctic Regions
Bacterial Proteins - chemistry
Calcium
Cold Temperature
Crystallography, X-Ray
Metalloendopeptidases - chemistry
Models, Molecular
protease
Pseudomonas aeruginosa - enzymology
psychrophilic enzymes
Sequence Homology, Amino Acid
Serine Endopeptidases
X‐ray crystallography
Zinc
Title Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile pseudomonas aeruginosa
URI https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fpro.5560061121
https://www.ncbi.nlm.nih.gov/pubmed/9385650
https://search.proquest.com/docview/79430697
https://pubmed.ncbi.nlm.nih.gov/PMC2143593
Volume 6
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Na9wwEB2SQGkupU0a6n6kOpT25F3L8peOy9IQCmmXkkJuZmRJzdJdefFmD0v_fEfyOumSQ6FHIbAHzcjzJL95A_Ahtyl6HblYSGvjTNucvoOSxzrHQmeqrFQQcb36Wlz-yL7c5DcHkA-1MIG036j5yC2WIze_DdzK1bIZDzyx8exqmvokL8X4EA5LIYYj-lAMydO-f3zB40oU1aDUmKS-uG2U-xRfEMrgx_BEiooATbKfkx4Bzcd8yb9xbEhEF8_h2Q5Bsklv6Qs4MO4ETieOTs_LLfvIAqczXJafwNPp0M_tFH7POrMIPby6LWu6LcHCBevlYzedYXrgxXhPsdYyQoYMF7_Q41AW5Bwo4TFfjhKmJu6O9gjZwAJhumtXt2Qtm63NRrcU27hmaLrNz7lr1_gSri8-X08v413rhbjJ_aWpwIQrXdFhJJNWG-6BoC65MaXKeVERLDLCSKVTiTZpMlRWFYYW1TRWK0RxBkeudeYVMBS2SjDDKi21F8qRpiGQkDVNhaVFriP4NKx9veoFNupeSjmlcVs_-CuC94Nralo6_2MDnWk369qL3CWFLCM46x11_6SdgyMo9zx4P-_FtfdnKOaCyPYuxiJIg6__YVs9-_7tYfT6v1_3Bo6DPm4odnwLRxQE5h2hnjt17nNOfh5i_Q_NEgTL
link.rule.ids 230,315,733,786,790,891,27957,27958,53827,53829
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9tAEB5RUAWXPqAI98UeqvZkx-_HMYqKAiU0qtKKm7VPgUjsyIkPgT_f2XUWmnJA7XE1kr27M-P5dj3zDcCnRIVU88i5UaGUGwuV4HewCFyR0FTELMuZIXEdXaTDn_HZZXK5BYmthTFJ-5xde9V05lXXVya3cj7jPZsn1huPBqEO8kXUewY76K9hZg_pthwyCLsO8mng5lGaW65GP9TlbV6ig3yKOCPYg-dFlCOk8Tej0iOo-Thj8k8ka0LRyUv4ZRfRZaDceO2Sefz2L37Hf17lK3ixBqek34lfw5as9uGgX-HBfLYin4lJFzX38PuwO7Ct4g7gbtzIqWkP1qwIb1aIOKekY6ZtG0mETbnRRkBqRRB0Ejq9oRriEsMUgbGU6EoXI-pXS3Q_nAMxudhNPb_CbSDjhWxFjW5DF4TKpsXl1gv6BiYnXyeDobvu6uDyRN_HRtQPmMjxnBMXSshAY0yRBVJmLAnSHBGXjGTBRFhQ5fOYMsVSidqSXAlGaXQI21VdySMgNFK5T2OKFiA0B08hOeKPmPOcZooGwoEvVqnlvOPuKDuW5hDHdflgCA4cW52XuHX6nwmtZN0uSs2f56dF5sBhZwH3T1pbjgPZhmncyzVv96YEFW34u9eKdSA0RvTE3Mrxj-8Po7f__bpj2B1ORufl-enFt3ewZ2h4TU3le9hGg5AfEFwt2UfjSr8Bj9sl2A
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BEdBLgT5EKFAfEJySzTvxcbV0VR4tESpSxSXyU626m6yym8O2f56xs2m77QGpx8hRYnvGmc_ON98AfEp0yIyOnBtRrd1Y6gS_gzRwZcJSGfMs51bE9fgkPfoTfz9Lzu6U-rKkfcEvvGoy9aqLc8utnE3FoOeJDYrjUWiCPI0GM6kHT-EZrtmQ9hv1PiUyCLsq8mng5lGa93qNfmhS3LzEBPoUsUawCc9plCOs8dcj0wO4-ZA1eRfN2nA0fgV_-4F0LJRLr11wT1zd03h81Ehfw9YKpJJhd8sbeKKqbdgZVrhBny7JZ2Jpo_Y8fhtejvqScTtwXTRqYsuENUsimiUizwnpFGrbRhHZU2-MM5BaEwSfhE0umYG6xCpGYEwlJuPFNg2rBS5D7AOxnOymnp3jVJBirlpZ4_Jhc8JU0-KQ6znbhdPx4enoyF1Vd3BFYs5lI-YHXOa434mpliowWFNmgVIZT4I0R-SlIkW5DCnTvogZ1zxVaDEltOSMRXuwUdWVeguERTr3WczyMJNGi4cqgTgkFiJnmWaBdOBLb9hy1ml4lJ1ac4jXdXnrDA4c9HYvcerMvxNWqbqdl0ZHz09p5sBe5wU3T1p5jwPZmnvctBv97vUWNLbV8V4Z14HQOtJ_-lYWv3_dXr179OsO4EXxdVz-_HbyYx82rRqvTa18DxvoD-oDYqwF_2hX0z-o7ChY
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Preliminary+crystal+structure+determination+of+the+alkaline+protease+from+the+Antarctic+psychrophile+Pseudomonas+aeruginosa&rft.jtitle=Protein+science&rft.au=Villeret%2C+V&rft.au=Chessa%2C+J+P&rft.au=Gerday%2C+C&rft.au=Van+Beeumen%2C+J&rft.date=1997-11-01&rft.issn=0961-8368&rft.eissn=1469-896X&rft.volume=6&rft.issue=11&rft.spage=2462&rft_id=info:doi/10.1002%2Fpro.5560061121&rft_id=info%3Apmid%2F9385650&rft.externalDocID=9385650
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0961-8368&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0961-8368&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0961-8368&client=summon