Development and application of a high throughput one-pot extraction protocol for quantitative LC-MS/MS analysis of phospholipids in serum and lipoprotein fractions in normolipidemic and dyslipidemic subjects

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1118-1119; pp. 137 - 147
• Main Authors: Gardner, Michael S., Kuklenyik, Zsuzsanna, Lehtikoski, Antony, Carter, Kayla A., McWilliams, Lisa G., Kusovschi, Jennifer, Bierbaum, Kevin, Jones, Jeffrey I., Rees, Jon, Reis, Gregory, Pirkle, James L., Barr, John R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.06.2019
• Subjects: blood serum; cholesterol; cholesteryl esters; Chromatography, High Pressure Liquid - methods; Dyslipidemias - blood; Dyslipidemias - diagnosis; high density lipoprotein; HILIC; Humans; Hydrophobic and Hydrophilic Interactions; Lipidomics; Lipids; Lipoproteins; liquid chromatography; liquid-liquid extraction; low density lipoprotein; lysophosphatidylcholine; phosphatidylethanolamines; phosphatidylinositols; Phospholipids; Phospholipids - blood; Phospholipids - isolation & purification; quantitative analysis; Reproducibility of Results; Sensitivity and Specificity; sphingomyelins; tandem mass spectrometry; Tandem Mass Spectrometry - methods; triacylglycerols; UHPLC-MS/MS; very low density lipoprotein; Lipids; Lipidomics; Lipoproteins; Phospholipids; UHPLC-MS/MS; HILIC

Progress toward better diagnosis and treatment of lipid metabolism-related diseases requires high throughput approaches for multiplexed quantitative analysis of structurally diverse lipids, including phospholipids (PLs). This work demonstrates a simplified “one-pot” phospholipid extraction protocol,...