Establishment and characterization of a new drug surviving cell line Am1010, derived directly from muscle metastases of a human lung adenocarcinoma patient with multi-drug-resistance to cisplatin, taxol, and gefitinib

• Published in: Acta pharmacologica Sinica Vol. 31; no. 5; pp. 601 - 608
• Main Authors: Li, Hui-ling, Xie, Si-ming, Zhang, Liang, Cai, Cheng-jie, Wang, Wei, Huang, Jun, Wang, Dao-yuan, Wen, Dan-ping, Deng, Qiu-hua, Zhong, Nan-shan, He, Jian-xing
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.05.2010; Nature Publishing Group
• Subjects: Adenocarcinoma - drug therapy; Adenocarcinoma - secondary; Adult; Animals; Biomedical and Life Sciences; Biomedicine; Cell Line, Tumor - cytology; Cell Line, Tumor - drug effects; Cell Line, Tumor - metabolism; Cell Line, Tumor - pathology; Cell Proliferation; Chromosomes - genetics; Cisplatin - pharmacology; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Female; Gene Expression Profiling; Humans; Immunology; Internal Medicine; Lung Neoplasms - drug therapy; Lung Neoplasms - secondary; Medical Microbiology; Mice; Mice, Nude; Original; original-article; Paclitaxel - pharmacology; Pharmacology/Toxicology; Quinazolines - pharmacology; Vaccine; multi-drug-resistance; Am1010; metastases; drug surviving cells; focal and cell adhesion; lung adenocarcinoma
• DOI: 10.1038/aps.2010.41
• ISSN: 1671-4083

Aim: To Characterize a new human lung cancer cell line Am1010, derived from drug-surviving cells (DSCs). Methods: The Am1010 cell line was established after 4 cycles of chemotherapy from an arm muscle metastasic tumor of a patient diagnosed with lung adenocarcinoma. The cell line has been remained in continuous culture for more than one year during this study. Results: The Am1010 cell line demonstrated in vitro multi-drug-resistance to cisplatin, taxol, and gefitinib. The Am1010 cell doubling time without drug treatment was 42.395 h. The IC 50 value of cisplatin was 4.299 μmol/L and >10 μmol/L for the Am1010 and P0318 (a cell line derived from non-DSCs) cells, respectively. The IC 50 value of taxol was 0.067 μmol/L and >1 μmol/L for the Am1010 and P0318 cells, respectively. The IC 50 value of gefitinib was 15.233 μmol/L and >70 μmol/L for Am1010 and P0318 cells, respectively. 11 genes involved in the focal adhesion and cell adhesion pathways were found to be differentially expressed. The cells of Am1010 have a significantly larger chromosome number than most lung cancer cell lines. Conclusion: This novel DSCs derived lung cancer cell line will be a valuable in vitro tool for the investigation of lung cancer drug resistance and metastasis.