Two-photon microscopic imaging of capillary red blood cell flux in mouse brain reveals vulnerability of cerebral white matter to hypoperfusion

• Published in: Journal of cerebral blood flow and metabolism Vol. 40; no. 3; pp. 501 - 512
• Main Authors: Li, Baoqiang, Ohtomo, Ryo, Thunemann, Martin, Adams, Stephen R, Yang, Jing, Fu, Buyin, Yaseen, Mohammad A, Ran, Chongzhao, Polimeni, Jonathan R, Boas, David A, Devor, Anna, Lo, Eng H, Arai, Ken, Sakadžić, Sava
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.03.2020
• Subjects: Animals; Capillaries - cytology; Capillaries - physiology; Cerebral Cortex; Cerebrovascular Circulation; Erythrocytes - cytology; Erythrocytes - physiology; Female; Gray Matter; Mice; Microscopy, Fluorescence, Multiphoton; Original; White Muscle Disease - blood; two-photon microscopy; hypoperfusion; capillary; RBC flux; White matter

Despite the importance of understanding the regulation of microvascular blood flow in white matter, no data on subcortical capillary blood flow parameters are available, largely due to the lack of appropriate imaging methods. To address this knowledge gap, we employed two-photon microscopy using a f...