Identification of 2-keto-4-methylthiobutyrate as an intermediate compound in methionine synthesis from 5'-methylthioadenosine

• Published in: The Journal of biological chemistry Vol. 257; no. 8; pp. 4196 - 4202
• Main Authors: Backlund, P S, Chang, C P, Smith, R A
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25.04.1982; American Society for Biochemistry and Molecular Biology
• Subjects: Adenosine - analogs & derivatives; Adenosine - metabolism; Animals; Deoxyadenosines; Kinetics; Liver - metabolism; Magnesium - pharmacology; Methionine - analogs & derivatives; Methionine - isolation & purification; Methionine - metabolism; Rats; Thionucleosides - metabolism

The synthesis of methionine from 5'-methylthioadenosine was examined in vitro using cell-free homogenates of rat liver. We had previously reported that methionine synthesized by this system contained carbons from the ribose moiety of 5'-methylthioadenosine (Backlund, P. S., Jr., and Smith,...