Identification of a Functional Vitamin D Response Element in the Murine Insig-2 Promoter and Its Potential Role in the Differentiation of 3T3-L1 Preadipocytes

• Published in: Molecular endocrinology (Baltimore, Md.) Vol. 19; no. 2; pp. 399 - 408
• Main Authors: Lee, Seunghee, Lee, Dong-Kee, Choi, Eunho, Lee, Jae W
• Format: Journal Article
• Language: English
• Published: United States Endocrine Society 01.02.2005; Oxford University Press
• Subjects: 3T3-L1 Cells; Adipocytes - cytology; Adipocytes - metabolism; Animals; Base Sequence; Bone Marrow Cells - cytology; Calcitriol - metabolism; Cell Differentiation; Dimerization; DNA Primers - chemistry; Dose-Response Relationship, Drug; Intracellular Signaling Peptides and Proteins - genetics; Ligands; Membrane Proteins - genetics; Mice; Models, Biological; Molecular Sequence Data; Plasmids - metabolism; PPAR gamma - metabolism; Promoter Regions, Genetic; Protein Binding; Receptors, Calcitriol - metabolism; Response Elements; Retinoid X Receptors - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transcriptional Activation; Transfection; Vitamin D - metabolism

Insulin-induced gene-1 (Insig-1) and its homolog Insig-2 encode closely related proteins of the endoplasmic reticulum that block proteolytic activation of sterol regulatory element binding proteins, membrane-bound transcription factors that activate synthesis of cholesterol and fatty acids in animal...