Characterization of Biochemical Properties of Bacillus subtilis RecQ Helicase

• Published in: Journal of bacteriology Vol. 196; no. 24; pp. 4216 - 4228
• Main Authors: Qin, Wei, Liu, Na-Nv, Wang, Lijun, Zhou, Min, Ren, Hua, Bugnard, Elisabeth, Liu, Jie-Lin, Zhang, Lin-Hu, Vendôme, Jeremie, Hu, Jin-Shan, Xi, Xu Guang
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.12.2014
• Subjects: Adenosine triphosphatase; Adenosine Triphosphatases - metabolism; adenosine triphosphate; Adenosine Triphosphate - metabolism; adenosinetriphosphatase; Bacillus subtilis; Bacillus subtilis - enzymology; bacteria; Bacteriology; Cell cycle; Deoxyribonucleic acid; DNA; DNA - metabolism; DNA damage; DNA helicases; DNA repair; DNA, Single-Stranded - metabolism; E coli; enzyme activity; Escherichia coli; genome; humans; Life Sciences; Magnesium - metabolism; Models, Molecular; Probiotics; Protein Binding; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; RecQ Helicases - metabolism; Saccharomyces cerevisiae; single-stranded DNA; Yeast

RecQ family helicases function as safeguards of the genome. Unlike Escherichia coli, the Gram-positive Bacillus subtilis bacterium possesses two RecQ-like homologues, RecQ[Bs] and RecS, which are required for the repair of DNA double-strand breaks. RecQ[Bs] also binds to the forked DNA to ensure a s...