Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor

• Published in: Journal of biomaterials science. Polymer ed. Vol. 11; no. 6; pp. 571 - 582
• Main Authors: Tabata, Yasuhiko, Ishii, Toshihiro, Muniruzzaman, Md, Hirano, Yoshiaki, Ikada, Yoshito
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Group 01.01.2000
• Subjects: ACTIVITY; Animals; BASIC; BIOLOGICAL; Cell Division - drug effects; Cell Line; COMPLEXATION; Cricetinae; DIGESTION; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; FACTOR; FIBROBLAST; Fibroblast Growth Factor 2 - chemistry; Fibroblast Growth Factor 2 - metabolism; GELATIN; Gelatin - chemistry; Gelatin - metabolism; GROWTH; Humans; Isoelectric Point; MOLECULAR; POLYION; Protein Binding; Recombinant Proteins - metabolism; Temperature; Time Factors; TRYPSIN; WEIGHT

The objective of this study is to investigate the influence of gelatin complexation on the biological activity of basic fibroblast growth factor (bFGF) and its resistance to trypsin digestion. When bFGF was mixed at 37°C with acidic gelatin with an isoelectric point(IEP)of 5.0, the activity to promo...