Screening for Small-Molecule Modulators of Long Noncoding RNA-Protein Interactions Using AlphaScreen

• Published in: Journal of biomolecular screening Vol. 20; no. 9; pp. 1132 - 1141
• Main Authors: Pedram Fatemi, Roya, Salah-Uddin, Sultan, Modarresi, Farzaneh, Khoury, Nathalie, Wahlestedt, Claes, Faghihi, Mohammad Ali
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.10.2015
• Subjects: Brain-Derived Neurotrophic Factor - biosynthesis; Brain-Derived Neurotrophic Factor - genetics; Drug Evaluation, Preclinical; Ellipticines - pharmacology; Enhancer of Zeste Homolog 2 Protein; Gene Expression - drug effects; HEK293 Cells; High-Throughput Screening Assays; Humans; Polycomb Repressive Complex 2 - biosynthesis; Polycomb Repressive Complex 2 - genetics; Protein Binding - drug effects; RNA Interference - drug effects; RNA, Long Noncoding - antagonists & inhibitors; RNA, Long Noncoding - biosynthesis; RNA, Long Noncoding - chemistry; brain-derived neurotrophic factor antisense (BDNFAS); epigenetic enzyme; RNA protein interaction; noncoding RNA; hox transcript antisense RNA (HOTAIR); AlphaScreen; brain-derived neurotrophic factor (BDNF); long noncoding RNA; Enhancer of zeste homolog 2 (EZH2); natural antisense transcript
• ISSN: 1087-0571; 2472-5552; 1552-454X
• DOI: 10.1177/1087057115594187

Long non–protein coding RNAs (lncRNAs) are an important class of molecules that help orchestrate key cellular events. Although their functional roles in cells are not well understood, thousands of lncRNAs and a number of possible mechanisms by which they act have been reported. LncRNAs can exert the...