Listeria monocytogenes mediated CFTR transgene transfer to mammalian cells

• Published in: The journal of gene medicine Vol. 4; no. 6; pp. 655 - 667
• Main Authors: Krusch, Stefan, Domann, Eugen, Frings, Monika, Zelmer, Andrea, Diener, Martin, Chakraborty, Trinad, Weiss, Siegfried
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.11.2002; Wiley Periodicals Inc
• Subjects: Animals; attenuation; bacterial vectors; Blotting, Western; CFTR; CHO Cells; Cricetinae; Cystic Fibrosis Transmembrane Conductance Regulator - genetics; Gene therapy; gene transfer; Gene Transfer Techniques; Genetic Vectors; Listeria monocytogenes - genetics; Microscopy, Confocal; Microscopy, Fluorescence; Patch-Clamp Techniques; Plasmids

Background Several approaches for gene therapy of cystic fibrosis using viral and non‐viral vectors are currently being undertaken. Nevertheless, the present data suggest that vectors currently being used will either have to be further modified or, alternatively, novel vector systems need to be deve...