Synthesis of cyclophosphamide metabolites by a peroxygenase from Marasmius rotula for toxicological studies on human cancer cells

• Published in: AMB Express Vol. 10; no. 1; pp. 128 - 13
• Main Authors: Steinbrecht, Susanne, Kiebist, Jan, König, Rosalie, Thiessen, Markus, Schmidtke, Kai-Uwe, Kammerer, Sarah, Küpper, Jan-Heiner, Scheibner, Katrin
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 18.07.2020; Springer Nature B.V; SpringerOpen
• Subjects: Acrolein; Bioactive compounds; Biocatalysis; Biocatalysts; Biocompatibility; Biological activity; Biomedical and Life Sciences; Biotechnology; Cancer; Cell viability; Chemical synthesis; Cyclophosphamide; Cytochrome P450; Cytochromes P450; Cytotoxicity; Drug development; Enzymes; Genotoxicity; Human drug metabolites; Hydrogen peroxide; Hydroxylation; Life Sciences; Liver; Marasmius; Metabolites; Microbial Genetics and Genomics; Microbiology; Mustard; Organic compounds; Original; Original Article; Peroxygenase; Toxicity; Toxicity testing; Tumor cell lines; Human drug metabolites; Cyclophosphamide; Biocatalysis; Peroxygenase; Toxicity

Cyclophosphamide (CPA) represents a widely used anti-cancer prodrug that is converted by liver cytochrome P450 (CYP) enzymes into the primary metabolite 4-hydroxycyclophosphamide (4-OH-CPA), followed by non-enzymatic generation of the bioactive metabolites phosphoramide mustard and acrolein. The use...