Genetic Reagents for Making Split-GAL4 Lines in Drosophila

• Published in: Genetics (Austin) Vol. 209; no. 1; pp. 31 - 35
• Main Authors: Dionne, Heather, Hibbard, Karen L, Cavallaro, Amanda, Kao, Jui-Chun, Rubin, Gerald M
• Format: Journal Article
• Language: English
• Published: United States Genetics Society of America 01.05.2018
• Subjects: Animals; Biological activity; Cell Line; Crosses, Genetic; Deoxyribonucleic acid; DNA; Drosophila; Drosophila - genetics; Drosophila melanogaster; Drosophila Proteins - chemistry; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Enhancer Elements, Genetic; Enhancers; Gene Expression; Genetic engineering; Genetic Vectors - genetics; Genetics; Genotype; Insects; Investigations; Kinases; Methods; Nervous system; Neurons; Neurosciences; Promoter Regions, Genetic; Reagents; Transcription factors; Transcription Factors - chemistry; Transcription Factors - genetics; Transcription Factors - metabolism; Transgenes; United States > US; neuron; enhancer; cell type; genetic intersection; gene expression

The ability to reproducibly target expression of transgenes to small, defined subsets of cells is a key experimental tool for understanding many biological processes. The nervous system contains thousands of distinct cell types and it has generally not been possible to limit expression to one or a f...