Active and Passive Demethylation of Male and Female Pronuclear DNA in the Mammalian Zygote

• Published in: Cell stem cell Vol. 15; no. 4; pp. 447 - 459
• Main Authors: Guo, Fan, Li, Xianlong, Liang, Dan, Li, Tong, Zhu, Ping, Guo, Hongshan, Wu, Xinglong, Wen, Lu, Gu, Tian-Peng, Hu, Boqiang, Walsh, Colum P., Li, Jinsong, Tang, Fuchou, Xu, Guo-Liang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 02.10.2014
• Subjects: 5-Methylcytosine - metabolism; Animals; Cell Nucleus - metabolism; Dioxygenases; DNA Methylation - genetics; DNA-Binding Proteins - metabolism; Female; Gene Deletion; Genetic Loci; Genome - genetics; Male; Mammals - genetics; Molecular Sequence Data; Oxidation-Reduction; Proto-Oncogene Proteins - metabolism; Reproducibility of Results; Reproduction - genetics; Sequence Analysis, DNA; Thymine DNA Glycosylase - metabolism; Zygote - metabolism

The epigenomes of mammalian sperm and oocytes, characterized by gamete-specific 5-methylcytosine (5mC) patterns, are reprogrammed during early embryogenesis to establish full developmental potential. Previous studies have suggested that the paternal genome is actively demethylated in the zygote whil...