Electrochemistry of cytochrome c immobilized on cardiolipin-modified electrodes: A probe for protein–lipid interactions

• Published in: Biochimica et biophysica acta Vol. 1830; no. 3; pp. 2798 - 2803
• Main Authors: Perhirin, Antoine, Kraffe, Edouard, Marty, Yanic, Quentel, François, Elies, Philippe, Gloaguen, Frederic
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2013; Elsevier
• Subjects: Analytical chemistry; Biochemistry, Molecular Biology; Biodiversity and Ecology; Carbon - chemistry; Cardiolipin; Cardiolipins - chemistry; Chemical Sciences; Cytochrome c; Cytochromes c - chemistry; Electrochemical Techniques; electrochemistry; Electrodes; Electron transfer; Electron Transport; Environmental Sciences; Fatty acid chain; fatty acids; glassy carbon electrode; Hydrophobic and Hydrophilic Interactions; hydrophobic bonding; Immobilized Proteins - chemistry; ionic strength; Kinetics; Life Sciences; Lipid anchorage; Microscopy, Atomic Force; Osmolar Concentration; Oxidation-Reduction; phosphatidylcholines; Phosphatidylcholines - chemistry; proteins; Surface Properties; Voltammetry; Cytochrome c; Electron transfer; Lipid anchorage; Voltammetry; Cardiolipin; Fatty acid chain

Electrochemistry of cytochrome c (cyt c) immobilized on a cardiolipin (CL)/phosphatidylcholine (PC) film supported on a glassy carbon electrode was investigated using variable-frequency AC voltammetry. At low ionic strength, we observed two redox-active subpopulations characterized by distinct value...