Muscle contraction induced arterial shear stress increases endothelial nitric oxide synthase phosphorylation in humans

• Published in: American journal of physiology. Heart and circulatory physiology Vol. 313; no. 4; pp. H854 - H859
• Main Authors: Casey, Darren P., Ueda, Kenichi, Wegman-Points, Lauren, Pierce, Gary L.
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.10.2017
• Series: Integrative Cardiovascular Physiology and Pathophysiology
• Subjects: Adult; Arteries - enzymology; Arteries - physiology; Brachial Artery - physiology; Endothelial cells; Exercise - physiology; Forearm; Forearm - physiology; Humans; Immunofluorescence; Male; Mechanical stimuli; Medical instruments; Muscle contraction; Muscle Contraction - physiology; Muscles; Muscular system; Nitric oxide; Nitric Oxide Synthase Type III - metabolism; Nitric-oxide synthase; Phosphorylation; Rapid Report; Regional Blood Flow - physiology; Rhythms; Shear rate; Shear stress; Stress, Physiological; Veins & arteries; Young Adult; endothelial nitric oxide synthase; exercise; shear stress; phosphorylation

We determined if local increases in brachial artery shear during repetitive muscle contractions induce changes in protein expression of endothelial nitric oxide synthase (eNOS) and/or phosphorylated (p-)eNOS at Ser 1177 , the primary activation site on eNOS, in endothelial cells (ECs) of humans. Sev...