The Application of Microsampling Disks in Circular Dichroism Spectroscopy for Peptide and Nucleic Acid Drugs
In recent years, there has been a growing focus on the development of medium-sized drugs based on peptides or nucleic acids owing to their potential therapeutic benefits. As some of these medium-sized drugs exert their therapeutic effects by adopting specific secondary structures, evaluating their c...
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| Published in | Chemical & pharmaceutical bulletin Vol. 72; no. 7; pp. 658 - 663 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
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Japan
The Pharmaceutical Society of Japan
11.07.2024
Japan Science and Technology Agency |
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| Abstract | In recent years, there has been a growing focus on the development of medium-sized drugs based on peptides or nucleic acids owing to their potential therapeutic benefits. As some of these medium-sized drugs exert their therapeutic effects by adopting specific secondary structures, evaluating their conformational states is crucial to ensure the efficacy, quality, and safety of the drug products. It is important to assess the structural integrity of biomolecular therapeutics to guarantee their intended pharmacological activity and maintain the required standards for drug development and manufacturing. One widely utilized technique for quality evaluation is secondary structural analysis using circular dichroism (CD) spectroscopy. Given the higher production and quality control costs associated with medium-sized drugs compared with small-molecule drugs, developing analytical techniques that enable CD analysis with reduced sample volumes is highly desirable. Herein, we focused on a microsampling disk-type cell as a potential solution for reducing the required sample volume. We investigated whether CD spectral analysis using a microsampling disk could provide equivalent spectra compared with the standard cell (sample volume: approx. 300 µL). Our findings demonstrated that the microsampling disk (sample volume: 2–10 µL) could be successfully applied to CD spectral analysis of peptide and nucleic acid drugs, paving the way for more efficient and cost-effective quality evaluation processes. |
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| AbstractList | In recent years, there has been a growing focus on the development of medium-sized drugs based on peptides or nucleic acids owing to their potential therapeutic benefits. As some of these medium-sized drugs exert their therapeutic effects by adopting specific secondary structures, evaluating their conformational states is crucial to ensure the efficacy, quality, and safety of the drug products. It is important to assess the structural integrity of biomolecular therapeutics to guarantee their intended pharmacological activity and maintain the required standards for drug development and manufacturing. One widely utilized technique for quality evaluation is secondary structural analysis using circular dichroism (CD) spectroscopy. Given the higher production and quality control costs associated with medium-sized drugs compared with small-molecule drugs, developing analytical techniques that enable CD analysis with reduced sample volumes is highly desirable. Herein, we focused on a microsampling disk-type cell as a potential solution for reducing the required sample volume. We investigated whether CD spectral analysis using a microsampling disk could provide equivalent spectra compared with the standard cell (sample volume: approx. 300 µL). Our findings demonstrated that the microsampling disk (sample volume: 2–10 µL) could be successfully applied to CD spectral analysis of peptide and nucleic acid drugs, paving the way for more efficient and cost-effective quality evaluation processes. In recent years, there has been a growing focus on the development of medium-sized drugs based on peptides or nucleic acids owing to their potential therapeutic benefits. As some of these medium-sized drugs exert their therapeutic effects by adopting specific secondary structures, evaluating their conformational states is crucial to ensure the efficacy, quality, and safety of the drug products. It is important to assess the structural integrity of biomolecular therapeutics to guarantee their intended pharmacological activity and maintain the required standards for drug development and manufacturing. One widely utilized technique for quality evaluation is secondary structural analysis using circular dichroism (CD) spectroscopy. Given the higher production and quality control costs associated with medium-sized drugs compared with small-molecule drugs, developing analytical techniques that enable CD analysis with reduced sample volumes is highly desirable. Herein, we focused on a microsampling disk-type cell as a potential solution for reducing the required sample volume. We investigated whether CD spectral analysis using a microsampling disk could provide equivalent spectra compared with the standard cell (sample volume: approx. 300 µL). Our findings demonstrated that the microsampling disk (sample volume: 2-10 µL) could be successfully applied to CD spectral analysis of peptide and nucleic acid drugs, paving the way for more efficient and cost-effective quality evaluation processes.In recent years, there has been a growing focus on the development of medium-sized drugs based on peptides or nucleic acids owing to their potential therapeutic benefits. As some of these medium-sized drugs exert their therapeutic effects by adopting specific secondary structures, evaluating their conformational states is crucial to ensure the efficacy, quality, and safety of the drug products. It is important to assess the structural integrity of biomolecular therapeutics to guarantee their intended pharmacological activity and maintain the required standards for drug development and manufacturing. One widely utilized technique for quality evaluation is secondary structural analysis using circular dichroism (CD) spectroscopy. Given the higher production and quality control costs associated with medium-sized drugs compared with small-molecule drugs, developing analytical techniques that enable CD analysis with reduced sample volumes is highly desirable. Herein, we focused on a microsampling disk-type cell as a potential solution for reducing the required sample volume. We investigated whether CD spectral analysis using a microsampling disk could provide equivalent spectra compared with the standard cell (sample volume: approx. 300 µL). Our findings demonstrated that the microsampling disk (sample volume: 2-10 µL) could be successfully applied to CD spectral analysis of peptide and nucleic acid drugs, paving the way for more efficient and cost-effective quality evaluation processes. |
| ArticleNumber | c24-00244 |
| Author | Misawa, Takashi Demizu, Yosuke Tsuji, Genichiro |
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| Cites_doi | 10.1016/j.omtn.2018.09.017 10.1007/s40265-013-0042-2 10.1039/C9CP05776E 10.1016/j.biochi.2017.10.006 10.1093/nar/gkad067 10.1517/13543784.16.11.1851 10.1038/s41589-023-01496-y 10.1039/D1SC00165E 10.1007/s40265-020-01269-0 10.1002/0471142700.nc0711s11 10.1038/s41565-021-00898-0 10.1136/dtb.2023.000007 10.1039/D3MD00487B 10.1016/j.bpj.2012.11.437 10.1021/jacs.3c03886 10.1016/j.addr.2023.114872 10.1016/S0014-4827(03)00257-X 10.1007/978-1-4939-9504-2_14 10.1007/978-1-4939-9504-2_11 |
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| References_xml | – reference: 10) Wang H., Dawber R. S., Zhang P., Walko M., Wilson A. J., Wang X., Chem. Sci. (Camb.), 12, 5977–5993 (2021). – reference: 19) Scott L. J., Drugs, 80, 335–339 (2020). – reference: 17) Hair P., Fiona Cameron F., McKeage K., Drugs, 73, 487–493 (2013). – reference: 8) Kawamoto Y., Wu Y., Takahashi Y., Takakura Y., Adv. Drug Deliv. Rev., 199, 114872 (2023). – reference: 1) Cheng J., Zhou J., Kong L., Wang H., Zhang Y., Wang X., Liu G., Chu Q., RSC Med. Chem., 14, 2496–2508 (2023). – reference: 22) Migliore M., Bonvicini A., Tognetti V., Guilhaudis L., Baaden M., Oulyadi H., Joubert L., Ségalas-Milazzo I., Phys. Chem. Chem. Phys., 22, 1611–1623 (2020). – reference: 11) Ministry of Health, Labour and Welfare. “Japanese Parmacopeia.”: ‹https://www.mhlw.go.jp/stf/seisakunitsuite/bunya/0000066597.html›, cited 16 April, 2024. – reference: 14) Wiśniewski K., Methods Mol. Biol., 2001, 235–271 (2019). – reference: 20) Watanabe N., Nagata T., Satou Y., Masuda S., Saito T., Kitagawa H., Komaki H., Takagaki K., Takeda S., Mol. Ther. Nucleic Acids, 13, 442–449 (2018). – reference: 7) Egli M., Manoharan M., Nucleic Acids Res., 51, 2529–2573 (2023). – reference: 15) Lexchin J., Mintzes B., Drug Ther. Bull., 61, 182–188 (2023). – reference: 23) Bishop G. R., Chaires J. B., Curr. Protoc. Nucleic Acid Chem., Chapter 7, 7.11.1–7.11.8 (2003). – reference: 2) Nakamura Y., Biochimie, 145, 22–33 (2018). – reference: 16) Weiss M. A., Vitam. Horm., 80, 33–49 (2009). – reference: 9) Nornes S., Growth C., Camp E., Ey P., Lardelli M., Exp. Cell Res., 289, 124–132 (2003). – reference: 21) “Supplement I to The Japanese Pharmacopoeia,” 18th edition, 2022. – reference: 4) Tanada M., Tamiya M., Matsuo A., et al., J. Am. Chem. Soc., 145, 16610–16620 (2023). – reference: 12) Kondo Y., Biophys. J., 104, 72a (2013). – reference: 18) Food and Drug Administration. “Highlights of Prescribing Information (Macugen).” 3–12 (July 2007): ‹https://pi.bauschhealth.com/globalassets/BHC/PI/Macugen-PI.pdf?ver=2021-05-21-022911-740›, cited 16 April, 2024. – reference: 5) Merz M. L., Habeshian S., Li B., David J. G. L., Nielsen A. L., Ji X., Il Khwildy K., Duany Benitez M. M., Phothirath P., Heinis C., Nat. Chem. Biol., 20, 624–633 (2024). – reference: 6) Kulkarni J. A., Witzigmann D., Thomson S. B., Chen S., Leavitt B. R., Cullis P. R., Meel R., Nat. Nanotechnol., 16, 630–643 (2021). – reference: 13) Wilson A. C., Meethal S. V., Bowen R. L., Atwood C. S., Expert Opin. Investig. Drugs, 16, 1851–1863 (2007). – reference: 3) Tsiamantas C., Otero–Ramirez M. E., Suga H., Methods Mol. Biol., 2001, 299–315 (2019). – ident: 18 – ident: 20 doi: 10.1016/j.omtn.2018.09.017 – ident: 17 doi: 10.1007/s40265-013-0042-2 – ident: 22 doi: 10.1039/C9CP05776E – ident: 2 doi: 10.1016/j.biochi.2017.10.006 – ident: 7 doi: 10.1093/nar/gkad067 – ident: 13 doi: 10.1517/13543784.16.11.1851 – ident: 5 doi: 10.1038/s41589-023-01496-y – ident: 10 doi: 10.1039/D1SC00165E – ident: 11 – ident: 19 doi: 10.1007/s40265-020-01269-0 – ident: 23 doi: 10.1002/0471142700.nc0711s11 – ident: 16 – ident: 6 doi: 10.1038/s41565-021-00898-0 – ident: 15 doi: 10.1136/dtb.2023.000007 – ident: 1 doi: 10.1039/D3MD00487B – ident: 12 doi: 10.1016/j.bpj.2012.11.437 – ident: 4 doi: 10.1021/jacs.3c03886 – ident: 8 doi: 10.1016/j.addr.2023.114872 – ident: 9 doi: 10.1016/S0014-4827(03)00257-X – ident: 3 doi: 10.1007/978-1-4939-9504-2_14 – ident: 14 doi: 10.1007/978-1-4939-9504-2_11 – ident: 21 |
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| SubjectTerms | Circular Dichroism circular dichroism (CD) spectra Cost analysis Dichroism Drug development Drugs Effectiveness medium-sized drug microsampling disk nucleic acid Nucleic acids Nucleic Acids - analysis Nucleic Acids - chemistry peptide Peptides Peptides - analysis Peptides - chemistry Product safety Quality assessment Quality control secondary structure Spectroscopic analysis Spectroscopy Spectrum analysis Structural analysis Structural integrity |
| Title | The Application of Microsampling Disks in Circular Dichroism Spectroscopy for Peptide and Nucleic Acid Drugs |
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