In vitro methods to determine the antioxidant activity of caffeic acid
Caffeic acid (CA) is a plant metabolite acting as a carcinogenic inhibitor, and exhibits a high antioxidant effect and some antimicrobial activity. Besides, this compound can be useful in the prevention of heart diseases and atherosclerosis, among others. The present study aims to determine the in v...
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| Published in | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 219; pp. 358 - 366 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier B.V
05.08.2019
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Caffeic acid (CA) is a plant metabolite acting as a carcinogenic inhibitor, and exhibits a high antioxidant effect and some antimicrobial activity. Besides, this compound can be useful in the prevention of heart diseases and atherosclerosis, among others. The present study aims to determine the in vitro antioxidant activity of CA in order to increase the frequency of its use and reliability in the prevention of damage caused by free radicals and other reactive species. The tests performed were as follows: Radical anion superoxide capture; crocin bleaching assay; capturing ability of hypochlorous acid; H2O2 capture; capturing capacity of the ABTS•+/DPPH•; and SOD-like activity. The values of the CA antioxidant activity were very close to the values of standards in all tests. Besides, CA presented an antioxidant activity greater than that of ascorbic acid and trolox, and its advantages include higher stability than ascorbic acid and extraction from natural sources, as opposed to trolox.
[Display omitted]
•Investigation of caffeic acid antioxidant activity.•Caffeic acid for prevention of damage caused by free radicals.•Caffeic acid antioxidant activity was very close to the standards in all tests.•Caffeic acid presented antioxidant activity greater then ascorbic acid and trolox. |
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| AbstractList | Caffeic acid (CA) is a plant metabolite acting as a carcinogenic inhibitor, and exhibits a high antioxidant effect and some antimicrobial activity. Besides, this compound can be useful in the prevention of heart diseases and atherosclerosis, among others. The present study aims to determine the in vitro antioxidant activity of CA in order to increase the frequency of its use and reliability in the prevention of damage caused by free radicals and other reactive species. The tests performed were as follows: Radical anion superoxide capture; crocin bleaching assay; capturing ability of hypochlorous acid; H
O
capture; capturing capacity of the ABTS
/DPPH
; and SOD-like activity. The values of the CA antioxidant activity were very close to the values of standards in all tests. Besides, CA presented an antioxidant activity greater than that of ascorbic acid and trolox, and its advantages include higher stability than ascorbic acid and extraction from natural sources, as opposed to trolox. Caffeic acid (CA) is a plant metabolite acting as a carcinogenic inhibitor, and exhibits a high antioxidant effect and some antimicrobial activity. Besides, this compound can be useful in the prevention of heart diseases and atherosclerosis, among others. The present study aims to determine the in vitro antioxidant activity of CA in order to increase the frequency of its use and reliability in the prevention of damage caused by free radicals and other reactive species. The tests performed were as follows: Radical anion superoxide capture; crocin bleaching assay; capturing ability of hypochlorous acid; H2O2 capture; capturing capacity of the ABTS•+/DPPH•; and SOD-like activity. The values of the CA antioxidant activity were very close to the values of standards in all tests. Besides, CA presented an antioxidant activity greater than that of ascorbic acid and trolox, and its advantages include higher stability than ascorbic acid and extraction from natural sources, as opposed to trolox. [Display omitted] •Investigation of caffeic acid antioxidant activity.•Caffeic acid for prevention of damage caused by free radicals.•Caffeic acid antioxidant activity was very close to the standards in all tests.•Caffeic acid presented antioxidant activity greater then ascorbic acid and trolox. |
| Author | Corrêa, Marcos Antonio Brunetti, Iguatemy Lourenço Isaac, Vera Lucia Borges Assis, Renata Pires Spagnol, Caroline Magnani Salgado, Hérida Regina Nunes |
| Author_xml | – sequence: 1 givenname: Caroline Magnani orcidid: 0000-0002-1329-8251 surname: Spagnol fullname: Spagnol, Caroline Magnani email: carol.magnani@hotmail.com organization: Department of Drugs and Medicines, São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, São Paulo 14800-903, Brazil – sequence: 2 givenname: Renata Pires surname: Assis fullname: Assis, Renata Pires email: renatapires_17@hotmail.com organization: Department of Clinical Analysis, São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, São Paulo 14800-903, Brazil – sequence: 3 givenname: Iguatemy Lourenço surname: Brunetti fullname: Brunetti, Iguatemy Lourenço email: brunetti@fcfar.unesp.br organization: Department of Clinical Analysis, São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, São Paulo 14800-903, Brazil – sequence: 4 givenname: Vera Lucia Borges surname: Isaac fullname: Isaac, Vera Lucia Borges email: veraisaac@fcfar.unesp.br organization: Department of Drugs and Medicines, São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, São Paulo 14800-903, Brazil – sequence: 5 givenname: Hérida Regina Nunes surname: Salgado fullname: Salgado, Hérida Regina Nunes email: salgadoh@fcfar.unesp.br organization: Department of Drugs and Medicines, São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, São Paulo 14800-903, Brazil – sequence: 6 givenname: Marcos Antonio surname: Corrêa fullname: Corrêa, Marcos Antonio email: correama@fcfar.unesp.br organization: Department of Drugs and Medicines, São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, São Paulo 14800-903, Brazil |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31055242$$D View this record in MEDLINE/PubMed |
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| Title | In vitro methods to determine the antioxidant activity of caffeic acid |
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