Nucleosome Positioning by an Evolutionarily Conserved Chromatin Remodeler Prevents Aberrant DNA Methylation in Neurospora

• Published in: Genetics (Austin) Vol. 211; no. 2; pp. 563 - 578
• Main Authors: Klocko, Andrew D, Uesaka, Miki, Ormsby, Tereza, Rountree, Michael R, Wiles, Elizabeth T, Adhvaryu, Keyur K, Honda, Shinji, Selker, Eric U
• Format: Journal Article
• Language: English
• Published: United States Genetics Society of America 01.02.2019
• Subjects: Adenosine triphosphatase; Androgens; Cancer; Chromatin; Chromatin - genetics; Chromatin - metabolism; Conserved Sequence; Cytosine; Defects; Deoxyribonucleic acid; DNA; DNA Methylation; Euchromatin; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungi; Gene expression; Gene Expression Regulation, Fungal; Genetics; Genomes; Heterochromatin; Histone Code; Histone H3; Insects; Investigations; Lysine; Methyltransferases - genetics; Methyltransferases - metabolism; Mutation; Neurospora; Neurospora - genetics; Nucleosomes; Nucleosomes - genetics; Nucleosomes - metabolism; Stem cells; Transcription; DNA methylation; nucleosome; Neurospora crassa; DIM-1; CATP; heterochromatin

In the filamentous fungus Neurospora crassa, constitutive heterochromatin is marked by tri-methylation of histone H3 lysine 9 (H3K9me3) and DNA methylation. We identified mutations in the Neurospora defective in methylation-1 (dim-1) gene that cause defects in cytosine methylation and implicate a pu...