Cholesterol Transport through Lysosome-Peroxisome Membrane Contacts

• Published in: Cell Vol. 161; no. 2; pp. 291 - 306
• Main Authors: Chu, Bei-Bei, Liao, Ya-Cheng, Qi, Wei, Xie, Chang, Du, Ximing, Wang, Jiang, Yang, Hongyuan, Miao, Hong-Hua, Li, Bo-Liang, Song, Bao-Liang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 09.04.2015
• Subjects: Adrenoleukodystrophy - metabolism; Amphotericin B - pharmacology; animal models; Animals; ATP-Binding Cassette Transporters - metabolism; Biological Transport; Cholesterol - metabolism; genes; Genome-Wide Association Study; Humans; low density lipoprotein cholesterol; lysosomes; Lysosomes - metabolism; Mice; patients; Peroxisomal Disorders - metabolism; Peroxisomal Disorders - pathology; Peroxisomes - metabolism; Phosphatidylinositol 4,5-Diphosphate - metabolism; RNA, Small Interfering - metabolism; Synaptotagmins - metabolism; Zebrafish

Cholesterol is dynamically transported among organelles, which is essential for multiple cellular functions. However, the mechanism underlying intracellular cholesterol transport has remained largely unknown. We established an amphotericin B-based assay enabling a genome-wide shRNA screen for delaye...