Species-Specific Peptide Ligands for the Detection of Bacillus anthracis Spores

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Published inApplied and Environmental Microbiology Vol. 69; no. 10; pp. 6288 - 6293
Main Authors WILLIAMS, David D, BENEDEK, Orsolya, TURNBOUGH, Charles L
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.10.2003
Subjects
Amino Acid Sequence
Anthrax
Bacillus anthracis - classification
Bacillus anthracis - genetics
Bacillus anthracis - isolation & purification
Bacillus anthracis - physiology
Bacteria
Base Sequence
Biological and medical sciences
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Ligands
Methods
Microbiology
Molecular Sequence Data
Peptide Library
Peptides
Peptides - metabolism
Species Specificity
Spores, Bacterial - metabolism
Bacillus anthracis
Analysis method
Bacillales
Peptides
Ligand
Bacteria
Bacillaceae
Detection
Spores
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Abstract Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to AEM .asm.org, visit: AEM       
AbstractList Currently available detectors for spores of Bacillus anthracis , the causative agent of anthrax, are inadequate for frontline use and general monitoring. There is a critical need for simple, rugged, and inexpensive detectors capable of accurate and direct identification of B. anthracis spores. Necessary components in such detectors are stable ligands that bind tightly and specifically to target spores. By screening a phage display peptide library, we identified a family of peptides, with the consensus sequence TYPXPXR, that bind selectively to B. anthracis spores. We extended this work by identifying a peptide variant, ATYPLPIR, with enhanced ability to bind to B. anthracis spores and an additional peptide, SLLPGLP, that preferentially binds to spores of species phylogenetically similar to, but distinct from, B. anthracis . These two peptides were used in tandem in simple assays to rapidly and unambiguously identify B. anthracis spores. We envision that these peptides can be used as sensors in economical and portable B. anthracis spore detectors that are essentially free of false-positive signals due to other environmental Bacillus spores.
Currently available detectors for spores of Bacillus anthracis, the causative agent of anthrax, are inadequate for frontline use and general monitoring. There is a critical need for simple, rugged, and inexpensive detectors capable of accurate and direct identification of B. anthracis spores. Necessary components in such detectors are stable ligands that bind tightly and specifically to target spores. By screening a phage display peptide library, we identified a family of peptides, with the consensus sequence TYPXPXR, that bind selectively to B. anthracis spores. We extended this work by identifying a peptide variant, ATYPLPIR, with enhanced ability to bind to B. anthracis spores and an additional peptide, SLLPGLP, that preferentially binds to spores of species phylogenetically similar to, but distinct from, B. anthracis. These two peptides were used in tandem in simple assays to rapidly and unambiguously identify B. anthracis spores. We envision that these peptides can be used as sensors in economical and portable B. anthracis spore detectors that are essentially free of false-positive signals due to other environmental Bacillus spores.
ABSTRACT Currently available detectors for spores of Bacillus anthracis , the causative agent of anthrax, are inadequate for frontline use and general monitoring. There is a critical need for simple, rugged, and inexpensive detectors capable of accurate and direct identification of B. anthracis spores. Necessary components in such detectors are stable ligands that bind tightly and specifically to target spores. By screening a phage display peptide library, we identified a family of peptides, with the consensus sequence TYPXPXR, that bind selectively to B. anthracis spores. We extended this work by identifying a peptide variant, ATYPLPIR, with enhanced ability to bind to B. anthracis spores and an additional peptide, SLLPGLP, that preferentially binds to spores of species phylogenetically similar to, but distinct from, B. anthracis . These two peptides were used in tandem in simple assays to rapidly and unambiguously identify B. anthracis spores. We envision that these peptides can be used as sensors in economical and portable B. anthracis spore detectors that are essentially free of false-positive signals due to other environmental Bacillus spores.
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Currently available detectors for spores of Bacillus anthracis, the causative agent of anthrax, are inadequate for frontline use and general monitoring. There is a critical need for simple, rugged, and inexpensive detectors capable of accurate and direct identification of B. anthracis spores. Necessary components in such detectors are stable ligands that bind tightly and specifically to target spores. By screening a phage display peptide library, we identified a family of peptides, with the consensus sequence TYPXPXR, that bind selectively to B. anthracis spores. We extended this work by identifying a peptide variant, ATYPLPIR, with enhanced ability to bind to B. anthracis spores and an additional peptide, SLLPGLP, that preferentially binds to spores of species phylogenetically similar to, but distinct from, B. anthracis. These two peptides were used in tandem in simple assays to rapidly and unambiguously identify B. anthracis spores. We envision that these peptides can be used as sensors in economical and portable B. anthracis spore detectors that are essentially free of false-positive signals due to other environmental Bacillus spores. [PUBLICATION ABSTRACT]
Author Orsolya Benedek
Charles L. Turnbough Jr
David D. Williams
AuthorAffiliation Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294
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Cites_doi 10.1139/m68-217
10.1016/S0956-5663(99)00039-1
10.1128/JB.185.5.1555-1563.2003
10.1111/j.1472-765X.1991.tb00608.x
10.1128/JB.181.8.2358-2362.1999
10.1128/AEM.69.5.2755-2764.2003
10.1038/nature01586
10.1128/JCM.40.8.2897-2902.2002
10.1016/S0958-1669(02)00282-3
10.1001/jama.287.17.2236
10.1016/S0009-8981(01)00629-5
10.1016/S0167-7012(03)00030-7
10.1146/annurev.micro.55.1.647
10.1056/NEJMra012892
10.1128/iai.39.1.371-376.1983
10.1128/MMBR.64.3.548-572.2000
10.1128/AEM.69.1.593-599.2003
10.1128/jb.177.12.3394-3406.1995
10.1001/jama.281.18.1735
10.3201/eid0706.010604
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Issue 10
Keywords Bacillus anthracis
Analysis method
Bacillales
Peptides
Ligand
Bacteria
Bacillaceae
Detection
Spores
Language English
License CC BY 4.0
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Notes Corresponding author. Mailing address: Department of Microbiology, University of Alabama at Birmingham, BBRB 409, 845 19th St. South, Birmingham, AL 35294. Phone: (205) 934-6289. Fax: (205) 975-5479. E-mail: ChuckT@uab.edu.
Present address: Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Pécs 7624, Hungary.
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e_1_3_2_20_2
e_1_3_2_21_2
e_1_3_2_22_2
e_1_3_2_23_2
e_1_3_2_24_2
e_1_3_2_25_2
(e_1_3_2_5_2) 1967; 26
(e_1_3_2_15_2) 2001; 314
e_1_3_2_9_2
e_1_3_2_8_2
e_1_3_2_16_2
e_1_3_2_7_2
e_1_3_2_17_2
e_1_3_2_6_2
e_1_3_2_18_2
e_1_3_2_19_2
e_1_3_2_10_2
e_1_3_2_11_2
e_1_3_2_4_2
e_1_3_2_12_2
e_1_3_2_3_2
e_1_3_2_13_2
e_1_3_2_2_2
e_1_3_2_14_2
References_xml – ident: e_1_3_2_14_2
  doi: 10.1139/m68-217
– ident: e_1_3_2_11_2
  doi: 10.1016/S0956-5663(99)00039-1
– ident: e_1_3_2_26_2
  doi: 10.1128/JB.185.5.1555-1563.2003
– ident: e_1_3_2_2_2
  doi: 10.1111/j.1472-765X.1991.tb00608.x
– ident: e_1_3_2_20_2
  doi: 10.1128/JB.181.8.2358-2362.1999
– ident: e_1_3_2_22_2
  doi: 10.1128/AEM.69.5.2755-2764.2003
– ident: e_1_3_2_23_2
  doi: 10.1038/nature01586
– ident: e_1_3_2_3_2
  doi: 10.1128/JCM.40.8.2897-2902.2002
– ident: e_1_3_2_4_2
  doi: 10.1016/S0958-1669(02)00282-3
– ident: e_1_3_2_7_2
– ident: e_1_3_2_10_2
  doi: 10.1001/jama.287.17.2236
– volume: 314
  start-page: 1
  year: 2001
  ident: e_1_3_2_15_2
  publication-title: Clin. Chim. Acta
  doi: 10.1016/S0009-8981(01)00629-5
– ident: e_1_3_2_27_2
  doi: 10.1016/S0167-7012(03)00030-7
– volume: 26
  start-page: 1504
  year: 1967
  ident: e_1_3_2_5_2
  publication-title: Fed. Proc.
– ident: e_1_3_2_17_2
  doi: 10.1146/annurev.micro.55.1.647
– ident: e_1_3_2_24_2
– ident: e_1_3_2_25_2
  doi: 10.1056/NEJMra012892
– ident: e_1_3_2_16_2
  doi: 10.1128/iai.39.1.371-376.1983
– ident: e_1_3_2_18_2
  doi: 10.1128/MMBR.64.3.548-572.2000
– ident: e_1_3_2_19_2
– ident: e_1_3_2_8_2
  doi: 10.1128/AEM.69.1.593-599.2003
– ident: e_1_3_2_6_2
  doi: 10.1128/jb.177.12.3394-3406.1995
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  doi: 10.1001/jama.281.18.1735
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  doi: 10.3201/eid0706.010604
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Snippet Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit...
Currently available detectors for spores of Bacillus anthracis, the causative agent of anthrax, are inadequate for frontline use and general monitoring. There...
ABSTRACT Currently available detectors for spores of Bacillus anthracis , the causative agent of anthrax, are inadequate for frontline use and general...
Currently available detectors for spores of Bacillus anthracis , the causative agent of anthrax, are inadequate for frontline use and general monitoring. There...
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SubjectTerms Amino Acid Sequence
Anthrax
Bacillus anthracis - classification
Bacillus anthracis - genetics
Bacillus anthracis - isolation & purification
Bacillus anthracis - physiology
Bacteria
Base Sequence
Biological and medical sciences
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Ligands
Methods
Microbiology
Molecular Sequence Data
Peptide Library
Peptides
Peptides - metabolism
Species Specificity
Spores, Bacterial - metabolism
Title Species-Specific Peptide Ligands for the Detection of Bacillus anthracis Spores
URI http://aem.asm.org/content/69/10/6288.abstract
https://www.ncbi.nlm.nih.gov/pubmed/14532093
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https://pubmed.ncbi.nlm.nih.gov/PMC201196
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