Tet and TDG Mediate DNA Demethylation Essential for Mesenchymal-to-Epithelial Transition in Somatic Cell Reprogramming

• Published in: Cell stem cell Vol. 14; no. 4; pp. 512 - 522
• Main Authors: Hu, Xiao, Zhang, Lei, Mao, Shi-Qing, Li, Zheng, Chen, Jiekai, Zhang, Run-Rui, Wu, Hai-Ping, Gao, Juan, Guo, Fan, Liu, Wei, Xu, Gui-Fang, Dai, Hai-Qiang, Shi, Yujiang Geno, Li, Xianlong, Hu, Boqiang, Tang, Fuchou, Pei, Duanqing, Xu, Guo-Liang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 03.04.2014
• Subjects: Animals; Blotting, Western; Cell Differentiation; Cell Lineage; Cells, Cultured; Cellular Reprogramming; DNA Glycosylases - physiology; DNA Methylation; DNA-Binding Proteins - physiology; Embryo, Mammalian - cytology; Embryo, Mammalian - metabolism; Embryonic Stem Cells - cytology; Embryonic Stem Cells - metabolism; Epigenesis, Genetic; Epithelial-Mesenchymal Transition; Fibroblasts - cytology; Fibroblasts - metabolism; Flow Cytometry; Gene Expression Regulation; Immunoenzyme Techniques; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Mice; Mice, Knockout; MicroRNAs - physiology; Proto-Oncogene Proteins - physiology; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics

Tet-mediated DNA oxidation is a recently identified mammalian epigenetic modification, and its functional role in cell-fate transitions remains poorly understood. Here, we derive mouse embryonic fibroblasts (MEFs) deleted in all three Tet genes and examine their capacity for reprogramming into induc...