Co-ordinate loss of protein kinase C and multidrug resistance gene expression in revertant MCF-7/Adr breast carcinoma cells

• Published in: British journal of cancer Vol. 75; no. 9; pp. 1330 - 1335
• Main Authors: BUDWORTH, J, GANT, T. W, GESCHER, A
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing Group 01.05.1997
• Subjects: Antineoplastic agents; ATP Binding Cassette Transporter, Subfamily B, Member 1 - biosynthesis; ATP Binding Cassette Transporter, Subfamily B, Member 1 - genetics; Biological and medical sciences; Breast Neoplasms - drug therapy; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; DNA Primers - chemistry; Doxorubicin - pharmacology; Drug Resistance, Multiple - genetics; Female; Gene Amplification; Gene Expression Regulation, Neoplastic; General aspects; Genes, MDR - genetics; Humans; Isoenzymes - biosynthesis; Medical sciences; Pharmacology. Drug treatments; Polymerase Chain Reaction; Protein Kinase C - biosynthesis; Protein Kinase C - genetics; RNA, Messenger - metabolism; Tumor Cells, Cultured; Adenocarcinoma; Antineoplastic agent; Human; Protein kinase C; Enzyme; Transferases; Cytotoxicity; Malignant tumor; Gene expression; Doxorubicin; In vitro; Mammary gland diseases; Antibiotic; Gene; Multiple resistance; Established cell line; Anthracyclins; Mammary gland; Negative therapeutic reaction

The aim of this study was to investigate the link between protein kinase C (PKC) and multidrug resistance (mdr) phenotype. The expression of both was studied in doxorubicin-resistant MCF-7/Adr cells as they reverted to the wild-type phenotype when cultured in the absence of drug. The following param...