Landscape and Dynamics of Single Immune Cells in Hepatocellular Carcinoma

• Published in: Cell Vol. 179; no. 4; pp. 829 - 845.e20
• Main Authors: Zhang, Qiming, He, Yao, Luo, Nan, Patel, Shashank J., Han, Yanjie, Gao, Ranran, Modak, Madhura, Carotta, Sebastian, Haslinger, Christian, Kind, David, Peet, Gregory W., Zhong, Guojie, Lu, Shuangjia, Zhu, Weihua, Mao, Yilei, Xiao, Mengmeng, Bergmann, Michael, Hu, Xueda, Kerkar, Sid P., Vogt, Anne B., Pflanz, Stefan, Liu, Kang, Peng, Jirun, Ren, Xianwen, Zhang, Zemin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 31.10.2019
• Subjects: ascites; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - immunology; Carcinoma, Hepatocellular - pathology; Cation Transport Proteins - genetics; Cell Communication - genetics; Cell Communication - immunology; Cell Lineage - genetics; Cell Lineage - immunology; dendritic cells; Dendritic Cells - immunology; Dendritic Cells - pathology; Gene Expression Regulation, Neoplastic; hepatoma; Humans; immunity; Inflammation - genetics; Inflammation - immunology; Inflammation - pathology; Leukocyte Common Antigens - immunology; ligands; liver; Liver - immunology; Liver - pathology; Liver Neoplasms - genetics; Liver Neoplasms - immunology; Liver Neoplasms - pathology; lymph nodes; Lymph Nodes - immunology; Lymph Nodes - pathology; lymphocytes; Lymphocytes - immunology; Lymphocytes - pathology; Lysosomal Membrane Proteins - genetics; macrophages; Macrophages - immunology; Macrophages - pathology; Membrane Glycoproteins - genetics; Myeloid Cells - immunology; Myeloid Cells - pathology; Neoplasm Proteins - genetics; patients; prognosis; sequence analysis; Sequence Analysis, RNA; Single-Cell Analysis; transcription (genetics); transcriptome; Transcriptome - genetics; Transcriptome - immunology; Tumor Microenvironment - genetics; Tumor Microenvironment - immunology

The immune microenvironment of hepatocellular carcinoma (HCC) is poorly characterized. Combining two single-cell RNA sequencing technologies, we produced transcriptomes of CD45+ immune cells for HCC patients from five immune-relevant sites: tumor, adjacent liver, hepatic lymph node (LN), blood, and...