Artemisinin activity-based probes identify multiple molecular targets within the asexual stage of the malaria parasites Plasmodium falciparum 3D7

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 113; no. 8; pp. 2080 - 2085
• Main Authors: Ismail, Hanafy M., Barton, Victoria, Phanchana, Matthew, Charoensutthivarakul, Sitthivut, Wong, Michael H. L., Hemingway, Janet, Biagini, Giancarlo A., O’Neill, Paul M., Ward, Stephen A.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 23.02.2016; National Acad Sciences
• Subjects: Antimalarials - chemical synthesis; Antimalarials - chemistry; Antimalarials - pharmacology; Antioxidants; Artemisinins - chemical synthesis; Artemisinins - chemistry; Artemisinins - pharmacology; Biological Sciences; Chromatography; Click Chemistry; Hemoglobin; Humans; Lactones - chemical synthesis; Lactones - chemistry; Lactones - pharmacology; Life Cycle Stages - drug effects; Liquid chromatography; Malaria; Molecular Probes - chemical synthesis; Molecular Probes - chemistry; Molecular Probes - pharmacology; Parasites; Plasmodium falciparum; Plasmodium falciparum - metabolism; Probes; Protein synthesis; Protozoan Proteins - antagonists & inhibitors; Protozoan Proteins - metabolism; Vector-borne diseases; artemisinin; chemical proteomics; antimalarial; molecular targets; bioactivation

The artemisinin (ART)-based antimalarials have contributed significantly to reducing global malaria deaths over the past decade, but we still do not know how they kill parasites. To gain greater insight into the potential mechanisms of ART drug action, we developed a suite of ART activity-based prot...