RisaAligner software for aligning fluorescence data between Agilent 2100 Bioanalyzer chips: Application to soil microbial community analysis

• Published in: BioTechniques Vol. 59; no. 6; pp. 347 - 358
• Main Authors: Navarro, Elisabeth, Fabrègue, Olivier, Scorretti, Riccardo, Reboulet, Jérémy, Simonet, Pascal, Dawson, Lorna, Demanèche, Sandrine
• Format: Journal Article
• Language: English
• Published: England Future Science Ltd 01.12.2015; Eaton Publishing; Taylor & Francis Group
• Subjects: Algorithms; alignment tool; DNA Fingerprinting - methods; DNA, Intergenic - chemistry; Electric power; Engineering Sciences; microbial ecology; Nonlinear Dynamics; Principal Component Analysis; RISA comparison; Software; Soil Microbiology; microbial ecology; RISA comparison; alignment tool

Ribosomal Intergenic Spacer Analysis (RISA) is a high-resolution and highly reproducible fingerprinting technique for discriminating between microbial communities. The community profiles can be visualized using the Agilent 2100 Bioanalyzer. Comparison between fingerprints relies upon precise estimat...