MR-seq: measuring a single cell’s transcriptome repeatedly by RNA-seq

• Published in: Science bulletin Vol. 62; no. 6; pp. 391 - 398
• Main Authors: Yang, Lu, Ma, Zhaochun, Cao, Chen, Zhang, Yuhao, Wu, Xinglong, Lee, Raymond, Hu, Boqiang, Wen, Lu, Ge, Hao, Huang, Yanyi, Lao, Kaiqin, Tang, Fuchou
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30.03.2017
• Subjects: Differentially expressed genes; gene expression; gene expression regulation; genes; Intra-embryonic heterogeneity; mice; Mouse early preimplantation embryos; sequence analysis; Single-cell RNA-seq; transcriptome; Mouse early preimplantation embryos; Differentially expressed genes; Single-cell RNA-seq; Intra-embryonic heterogeneity

We described a novel single-cell RNA-seq technique called MR-seq (measure a single-cell transcriptome repeatedly), which permits statistically assessing the technical variation and identifying the differentially expressed genes between just two single cells by measuring each single cell twice. We de...