Endocytosis of NBD‐Sphingolipids in Neurons: Exclusion from Degradative Compartments and Transport to the Golgi Complex

• Published in: Traffic (Copenhagen, Denmark) Vol. 2; no. 6; pp. 395 - 405
• Main Authors: Babià, Teresa, Ledesma, Maria Dolores, Saffrich, Rainer, Kok, Jan Willem, Dotti, Carlos G., Egea, Gustavo
• Format: Journal Article
• Language: English
• Published: Oxford, UK Munksgaard International Publishers 01.06.2001
• Subjects: Animals; Antineoplastic Agents - pharmacology; Brefeldin A - pharmacology; Cell Differentiation; Cell Line; Cell Membrane - metabolism; Cells, Cultured; Chromatography, Thin Layer; Cytoplasm - metabolism; Endocytosis; Endosomes - metabolism; Glucosylceramides - biosynthesis; Golgi Apparatus - metabolism; Golgi complex; Hippocampus - cytology; Hippocampus - metabolism; lipid trafficking; Lysosomes - metabolism; Microscopy, Phase-Contrast; NBD‐sphingolipids; neurons; Neurons - metabolism; Nocodazole - pharmacology; Protein Synthesis Inhibitors - pharmacology; Rats; Sphingolipids - biosynthesis; Sphingomyelins - metabolism; Temperature; Time Factors; Transferrin - metabolism

Sphingolipids are abundant constituents of neuronal membranes that have been implicated in intracellular signaling, neurite outgrowth and differentiation. Differential localization and trafficking of lipids to membrane domains contribute to the specialized functions. In non‐neuronal cultured cell li...