Molecular detection and isolation of facultatively methylotrophic bacteria, including Methylobacterium podarium sp. nov., from the human foot microflora

• Published in: Environmental microbiology Vol. 6; no. 8; pp. 820 - 830
• Main Authors: Anesti, Vasiliki, Vohra, Jyotsna, Goonetilleka, Shalini, McDonald, Ian R., Sträubler, Bettina, Stackebrandt, Erko, Kelly, Donovan P., Wood, Ann P.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.08.2004
• Subjects: Adult; Alcohol Oxidoreductases - genetics; Bacteria - cytology; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; Bacterial Proteins - genetics; Biodiversity; Brevibacterium; Brevibacterium - genetics; Brevibacterium - isolation & purification; Brevibacterium - metabolism; DNA, Bacterial - analysis; DNA, Bacterial - chemistry; DNA, Bacterial - isolation & purification; DNA, Ribosomal - chemistry; DNA, Ribosomal - isolation & purification; Ecosystem; Female; Foot - microbiology; Genes, Bacterial - genetics; Genes, rRNA - genetics; Humans; Hyphomicrobium - genetics; Hyphomicrobium methylovorum; Male; Methylamines - metabolism; Methylobacterium - cytology; Methylobacterium - genetics; Methylobacterium - isolation & purification; Methylobacterium - metabolism; Methylobacterium extorquens; Middle Aged; Molecular Sequence Data; Phylogeny; Pseudomonas; Pseudomonas - genetics; Pseudomonas - isolation & purification; Pseudomonas - metabolism; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sulfhydryl Compounds - metabolism; Sulfides - metabolism

Summary This is the first study to demonstrate that diverse methylotrophic bacteria occur in the human foot microflora. Polymerase chain reaction (PCR) amplification of DNA from the soles and toe clefts of feet of five subjects indicated Methylobacterium strains to be present in all cases. Polymeras...