Karnovsky’s fixative prevents artifacts appearing as vacuolation derived from tissue processing in kidneys treated with antisense oligonucleotide

• Published in: Journal of Toxicologic Pathology Vol. 34; no. 4; pp. 367 - 371
• Main Authors: Nishina, Hironobu, Sakairi, Tetsuya, Kashimura, Akane, Sato, Hiroko, Mizukawa, Mao, Ozawa, Yuhei, Nishikawa, Satomi
• Format: Journal Article
• Language: English
• Published: Tokyo JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY 01.10.2021; Japan Science and Technology Agency; Japanese Society of Toxicologic Pathology
• Subjects: Accumulation; antisense oligonucleotide; Antisense oligonucleotides; Antisense therapy; basophilic granule; Buffers; Fixation; Granular materials; Karnovsky’s fixative; kidney; Kidneys; Morphology; Nucleic acids; Oligonucleotides; Proximal tubules; Tissues; vacuolation; Vacuoles

Antisense oligonucleotide (ASO) therapies have been identified as a new treatment modality for intractable diseases. In kidneys treated with ASOs, vacuoles, in addition to basophilic granules, are often observed in the proximal tubules. Some reports have described that these vacuoles are likely to b...