Genetic and Structural Characterization of L11 Lipooligosaccharide from Neisseria meningitidis Serogroup A Strains

• Published in: The Journal of biological chemistry Vol. 285; no. 26; pp. 19874 - 19883
• Main Authors: Mistretta, Noëlle, Seguin, Delphine, Thiébaud, Jerôme, Vialle, Sandrine, Blanc, Frédéric, Brossaud, Marina, Talaga, Philippe, Norheim, Gunnstein, Moreau, Monique, Rokbi, Bachra
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25.06.2010; American Society for Biochemistry and Molecular Biology
• Subjects: Bacteria; Bacterial Genetics; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Binding Sites; Carbohydrate Sequence; Carbohydrate Structure; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Electrophoresis, Polyacrylamide Gel; Gas Chromatography-Mass Spectrometry; Gel Electrophoresis; Gene Amplification; Gene Expression; Glycosylation; Glycosyltransferases - genetics; Glycosyltransferases - metabolism; Humans; Lipopolysaccharide (LPS); Lipopolysaccharides - biosynthesis; Lipopolysaccharides - chemistry; Magnetic Resonance Spectroscopy; Meningitis, Meningococcal - microbiology; Microbiology; Molecular Sequence Data; Molecular Structure; Monosaccharides - analysis; N-Acetylglucosaminyltransferases - genetics; N-Acetylglucosaminyltransferases - metabolism; Neisseria meningitidis; Neisseria meningitidis, Serogroup A - classification; Neisseria meningitidis, Serogroup A - genetics; Neisseria meningitidis, Serogroup A - metabolism; Sequence Analysis, DNA; Serotyping; Species Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Gene Expression; Gene Amplification; Gel Electrophoresis; Bacteria; Bacterial Genetics; Carbohydrate Structure; Lipopolysaccharide (LPS)

The lipooligosaccharide (LOS) of immunotype L11 is unique within serogroup A meningococci. In order to resolve its molecular structure, we conducted LOS genotyping by PCR analysis of genes responsible for α-chain sugar addition (lgtA, -B, -C, -E, -H, and -F) and inner core substituents (lgtG, lpt-3,...