Leukocyte function assessed via serial microlitre sampling of peripheral blood from sepsis patients correlates with disease severity

• Published in: Nature biomedical engineering Vol. 3; no. 12; pp. 961 - 973
• Main Authors: Jundi, Bakr, Ryu, Hyunryul, Lee, Do-Hyun, Abdulnour, Raja-Elie E., Engstrom, Braden D., Duvall, Melody G., Higuera, Angelica, Pinilla-Vera, Mayra, Benson, Maura E., Lee, Jaemyon, Krishnamoorthy, Nandini, Baron, Rebecca M., Han, Jongyoon, Voldman, Joel, Levy, Bruce D.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.12.2019; Nature Publishing Group
• Subjects: 13/31; 13/62; 14; 14/35; 631/250; 692/420; 692/699; Adult; Aged; Aged, 80 and over; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Blood; CD16 antigen; Cell activation; Counting; Cross-Sectional Studies; Elastase; Female; GPI-Linked Proteins; Humans; Information systems; Leukocyte Count; Leukocyte Elastase - blood; Leukocytes; Leukocytes (neutrophilic); Male; Microfluidic Analytical Techniques - instrumentation; Microfluidic Analytical Techniques - methods; Microfluidics; Middle Aged; Monocytes; Neutrophils; Parameters; Peripheral blood; Phenotype; Phenotypes; Receptors, IgG; Sampling; Sepsis; Sepsis - blood; Sepsis - diagnosis; Severity of Illness Index; Young Adult

Dysregulated leukocyte responses underlie the pathobiology of sepsis, which is a leading cause of death. However, measures of leukocyte function are not routinely available in clinical care. Here we report the development and testing of an inertial microfluidic system for the label-free isolation an...