Leukocyte function assessed via serial microlitre sampling of peripheral blood from sepsis patients correlates with disease severity

Dysregulated leukocyte responses underlie the pathobiology of sepsis, which is a leading cause of death. However, measures of leukocyte function are not routinely available in clinical care. Here we report the development and testing of an inertial microfluidic system for the label-free isolation an...

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Published inNature biomedical engineering Vol. 3; no. 12; pp. 961 - 973
Main Authors Jundi, Bakr, Ryu, Hyunryul, Lee, Do-Hyun, Abdulnour, Raja-Elie E., Engstrom, Braden D., Duvall, Melody G., Higuera, Angelica, Pinilla-Vera, Mayra, Benson, Maura E., Lee, Jaemyon, Krishnamoorthy, Nandini, Baron, Rebecca M., Han, Jongyoon, Voldman, Joel, Levy, Bruce D.
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Published London Nature Publishing Group UK 01.12.2019
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Abstract Dysregulated leukocyte responses underlie the pathobiology of sepsis, which is a leading cause of death. However, measures of leukocyte function are not routinely available in clinical care. Here we report the development and testing of an inertial microfluidic system for the label-free isolation and downstream functional assessment of leukocytes from 50 μl of peripheral blood. We used the system to assess leukocyte phenotype and function in serial samples from 18 hospitalized patients with sepsis and 10 healthy subjects. The sepsis samples had significantly higher levels of CD16 dim and CD16 − neutrophils and CD16 + ‘intermediate’ monocytes, as well as significantly lower levels of neutrophil-elastase release, O 2 − production and phagolysosome formation. Repeated sampling of sepsis patients over 7 days showed that leukocyte activation (measured by isodielectric separation) and leukocyte phenotype and function were significantly more predictive of the clinical course than complete-blood-count parameters. We conclude that the serial assessment of leukocyte function in microlitre blood volumes is feasible and that it provides significantly more prognostic information than leukocyte counting. The serial assessment of the functional parameters of leukocytes isolated via an inertial microfluidic system from 50 μl of peripheral blood from sepsis patients provides significantly more prognostic information than leukocyte counting.
AbstractList Dysregulated leukocyte responses underlie the pathobiology of sepsis, which is a leading cause of death. However, measures of leukocyte function are not routinely available in clinical care. Here we report the development and testing of an inertial microfluidic system for the label-free isolation and downstream functional assessment of leukocytes from 50 μl of peripheral blood. We used the system to assess leukocyte phenotype and function in serial samples from 18 hospitalized patients with sepsis and 10 healthy subjects. The sepsis samples had significantly higher levels of CD16dim and CD16− neutrophils and CD16+ ‘intermediate’ monocytes, as well as significantly lower levels of neutrophil-elastase release, O2− production and phagolysosome formation. Repeated sampling of sepsis patients over 7 days showed that leukocyte activation (measured by isodielectric separation) and leukocyte phenotype and function were significantly more predictive of the clinical course than complete-blood-count parameters. We conclude that the serial assessment of leukocyte function in microlitre blood volumes is feasible and that it provides significantly more prognostic information than leukocyte counting.The serial assessment of the functional parameters of leukocytes isolated via an inertial microfluidic system from 50 μl of peripheral blood from sepsis patients provides significantly more prognostic information than leukocyte counting.
Dysregulated leukocyte responses underlie the pathobiology of sepsis, which is a leading cause of death. However, measures of leukocyte function are not routinely available in clinical care. Here we report the development and testing of an inertial microfluidic system for the label-free isolation and downstream functional assessment of leukocytes from 50 μl of peripheral blood. We used the system to assess leukocyte phenotype and function in serial samples from 18 hospitalized patients with sepsis and 10 healthy subjects. The sepsis samples had significantly higher levels of CD16 dim and CD16 − neutrophils and CD16 + ‘intermediate’ monocytes, as well as significantly lower levels of neutrophil-elastase release, O 2 − production and phagolysosome formation. Repeated sampling of sepsis patients over 7 days showed that leukocyte activation (measured by isodielectric separation) and leukocyte phenotype and function were significantly more predictive of the clinical course than complete-blood-count parameters. We conclude that the serial assessment of leukocyte function in microlitre blood volumes is feasible and that it provides significantly more prognostic information than leukocyte counting. The serial assessment of the functional parameters of leukocytes isolated via an inertial microfluidic system from 50 μl of peripheral blood from sepsis patients provides significantly more prognostic information than leukocyte counting.
Dysregulated leukocyte responses underlie the pathobiology of sepsis, which is a leading cause of death. However, measures of leukocyte function are not routinely available in clinical care. Here we report the development and testing of an inertial microfluidic system for the label-free isolation and downstream functional assessment of leukocytes from 50 μl of peripheral blood. We used the system to assess leukocyte phenotype and function in serial samples from 18 hospitalized patients with sepsis and 10 healthy subjects. The sepsis samples had significantly higher levels of CD16 and CD16 neutrophils and CD16 'intermediate' monocytes, as well as significantly lower levels of neutrophil-elastase release, O production and phagolysosome formation. Repeated sampling of sepsis patients over 7 days showed that leukocyte activation (measured by isodielectric separation) and leukocyte phenotype and function were significantly more predictive of the clinical course than complete-blood-count parameters. We conclude that the serial assessment of leukocyte function in microlitre blood volumes is feasible and that it provides significantly more prognostic information than leukocyte counting.
Author Han, Jongyoon
Lee, Do-Hyun
Baron, Rebecca M.
Benson, Maura E.
Abdulnour, Raja-Elie E.
Krishnamoorthy, Nandini
Jundi, Bakr
Engstrom, Braden D.
Lee, Jaemyon
Ryu, Hyunryul
Pinilla-Vera, Mayra
Duvall, Melody G.
Voldman, Joel
Levy, Bruce D.
Higuera, Angelica
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Snippet Dysregulated leukocyte responses underlie the pathobiology of sepsis, which is a leading cause of death. However, measures of leukocyte function are not...
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SubjectTerms 13/31
13/62
14
14/35
631/250
692/420
692/699
Adult
Aged
Aged, 80 and over
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Blood
CD16 antigen
Cell activation
Counting
Cross-Sectional Studies
Elastase
Female
GPI-Linked Proteins
Humans
Information systems
Leukocyte Count
Leukocyte Elastase - blood
Leukocytes
Leukocytes (neutrophilic)
Male
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microfluidics
Middle Aged
Monocytes
Neutrophils
Parameters
Peripheral blood
Phenotype
Phenotypes
Receptors, IgG
Sampling
Sepsis
Sepsis - blood
Sepsis - diagnosis
Severity of Illness Index
Young Adult
Title Leukocyte function assessed via serial microlitre sampling of peripheral blood from sepsis patients correlates with disease severity
URI https://link.springer.com/article/10.1038/s41551-019-0473-5
https://www.ncbi.nlm.nih.gov/pubmed/31712645
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https://www.proquest.com/docview/3143078986
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https://pubmed.ncbi.nlm.nih.gov/PMC6899180
Volume 3
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