The alpha-subunit of the Na,K-ATPase has catalytic activity independent of the beta-subunit

• Published in: The Journal of biological chemistry Vol. 269; no. 38; pp. 23420 - 23425
• Main Authors: Blanco, G, DeTomaso, A W, Koster, J, Xie, Z J, Mercer, R W
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 23.09.1994
• Subjects: Animals; Baculoviridae; Catalysis; Dogs; Egtazic Acid - pharmacology; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines - pharmacology; Moths; Recombinant Proteins; Rodentia; Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors; Sodium-Potassium-Exchanging ATPase - chemistry; Sodium-Potassium-Exchanging ATPase - metabolism; Vanadates - pharmacology

All catalytic activities of the Na,K-ATPase have been ascribed to the alpha-subunit; however, normal activity requires the presence of the beta-subunit. Using recombinant baculoviruses to infect insect cells, we demonstrate that the alpha-subunit, without the beta-subunit, has catalytic activity. Du...