Deletion of the KU70 homologue facilitates gene targeting in Lipomyces starkeyi strain NRRL Y-11558

• Published in: Current genetics Vol. 65; no. 1; pp. 269 - 282
• Main Authors: Dai, Ziyu, Pomraning, Kyle R., Deng, Shuang, Hofstad, Beth A., Panisko, Ellen A., Rodriguez, Diana, Butcher, Mark G., Culley, David E., Magnuson, Jon K.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2019; Springer Nature B.V
• Subjects: beta-glucuronidase; Biochemistry; Biodegradation; Biomedical and Life Sciences; Cell Biology; DNA Breaks, Double-Stranded - radiation effects; DNA End-Joining Repair - genetics; Efficiency; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gamma Rays; Gene Deletion; Gene expression; Gene Expression Regulation, Fungal; gene overexpression; Gene sequencing; Gene targeting; Genes; Glycogen; Homologous recombination; Homology; Insertion; Ku Autoantigen - genetics; Ku Autoantigen - metabolism; Life Sciences; Lipids; Lipids - biosynthesis; Lipomyces - classification; Lipomyces - genetics; Lipomyces - metabolism; Lipomyces starkeyi; Loci; Microbial Genetics and Genomics; Microbiology; Mutagenesis, Site-Directed; Non-homologous end joining; Original Article; Overexpression; Plant Sciences; Proteomics; Reporter gene; reporter genes; Synthesis; Ultraviolet Rays; Yeast; yeasts; Non-homologous end-joining (NHEJ); Oleaginous yeast; Homologous recombination; Ls; Lipid production and degradation; LsKU70; Lipomyces starkeyi

The objective of this study was to disrupt the non-homologous end-joining (NHEJ) pathway gene (Ls ku70 Δ) and evaluate the effects of selected gene deletions related to glycogen synthesis (Ls GSY1 ) and lipid degradation (Ls MFE1 , Ls PEX10 , and Ls TGL4 ) on lipid production in the oleaginous yeast...