Display of cellulases on the cell surface of Saccharomyces cerevisiae for high yield ethanol production from high-solid lignocellulosic biomass

• Published in: Bioresource technology Vol. 108; pp. 128 - 133
• Main Authors: Matano, Yuki, Hasunuma, Tomohisa, Kondo, Akihiko
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2012
• Subjects: Aspergillus; Aspergillus - enzymology; beta-Glucosidase; Bioethanol; Biomass; Cell-surface display; Cellulase; Cellulases; Cellulases - metabolism; cellulose; Cellulose 1,4-beta-Cellobiosidase; distillation; DNA Primers; DNA Primers - genetics; endo-1,4-beta-glucanase; energy; engineering; enzymology; ethanol; Ethanol - metabolism; ethanol production; Fermentation; genetics; Lignin; Lignin - metabolism; Lignocellulose; Membrane Proteins; Membrane Proteins - metabolism; metabolism; methods; Oryza sativa; Plasmids; Plasmids - genetics; Polymerase Chain Reaction; Protein Engineering; Protein Engineering - methods; rice straw; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; sugars; yeasts; Cell-surface display; Bioethanol; Biomass; Lignocellulose; Cellulase

► Economically feasible ethanol production process requires high-titer ethanol. ► High-solid cellulosic materials were fermented using a cellulase-displaying yeast. ► Pretreated biomass was converted to ethanol with 89% theoretical yield within 72h. ► Cell-surface engineering successfully reduced co...