Akt-Mediated Phosphorylation of Argonaute 2 Downregulates Cleavage and Upregulates Translational Repression of MicroRNA Targets

• Published in: Molecular cell Vol. 50; no. 3; pp. 356 - 367
• Main Authors: Horman, Shane R., Janas, Maja M., Litterst, Claudia, Wang, Bingbing, MacRae, Ian J., Sever, Mary J., Morrissey, David V., Graves, Paul, Luo, Biao, Umesalma, Shaikamjad, Qi, Hank H., Miraglia, Loren J., Novina, Carl D., Orth, Anthony P.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 09.05.2013
• Subjects: Argonaute Proteins - genetics; Argonaute Proteins - metabolism; Cell Line; Cell Line, Tumor; Down-Regulation; HEK293 Cells; HeLa Cells; Humans; messenger RNA; microRNA; MicroRNAs - genetics; Phosphorylation; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; proto-oncogenes; RNA interference; translation (genetics); Up-Regulation

A high-throughput RNA interference (RNAi) screen targeting 542 genes of the human kinome was used to discover regulators of RNAi. Here we report that the proto-oncogene Akt-3/PKBγ (Akt3) phosphorylates Argonaute 2 (Ago2) at S387, which downregulates cleavage and upregulates translational repression...