Susceptibility of Mycobacterium tuberculosis to first-line antimycobacterial agents in a Brazilian hospital: assessing the utility of the tetrazolium (MTT) microplate assay

We conducted a cross-sectional, hospital-based study between January 2006-March 2008 to estimate the resistance of Mycobacterium tuberculosis to first-line drugs in patients with tuberculosis at a Brazilian hospital. We evaluated the performance of the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetr...

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Published inMemórias do Instituto Oswaldo Cruz Vol. 105; no. 5; pp. 661 - 664
Main Authors Ferrari, Michela De Luca, Telles, Maria Alice da Silva, Ferrazoli, Lucilaine, Levy, Carlos Emílio, Villares, Maria Cecília Barison, Moretti, Maria Luiza, Resende, Mariângela Ribeiro
Format Journal Article
LanguageEnglish
Published Brazil Instituto Oswaldo Cruz, Ministério da Saúde 01.08.2010
Fundação Oswaldo Cruz (FIOCRUZ)
Subjects
Adult
Anti-Bacterial Agents - pharmacology
Coloring Agents
colourimetric method
Cross-Sectional Studies
drug susceptibility test
Female
hospital
Humans
Male
Microbial Sensitivity Tests - methods
Mycobacterium tuberculosis
Mycobacterium tuberculosis - drug effects
Mycobacterium tuberculosis - isolation & purification
PARASITOLOGY
resistance
Retrospective Studies
tetrazolium
Tetrazolium Salts
Thiazoles
TROPICAL MEDICINE
tuberculosis
Tuberculosis - microbiology
Tuberculosis, Multidrug-Resistant - microbiology
tetrazolium
drug susceptibility test
colourimetric method
hospital
resistance
tuberculosis
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Abstract We conducted a cross-sectional, hospital-based study between January 2006-March 2008 to estimate the resistance of Mycobacterium tuberculosis to first-line drugs in patients with tuberculosis at a Brazilian hospital. We evaluated the performance of the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] (MTT) microplate assay compared with the Bactec-MGIT 960 system for mycobacteria testing. The prevalence of resistance in M. tuberculosis was 6.7%. Multidrug-resistance [resistance to rifampicin (RMP) and isoniazid (INH)], INH-resistance and streptomycin (SM)-resistance accounted for 1%, 3.8% and 3.8% of all resistance, respectively, and all isolates were susceptible to ethambutol (EM). The resistance was primary in four cases and acquired in three cases and previous treatment was associated with resistance (p = 0.0129). Among the 119 M. tuberculosis isolates, complete concordance of the results for INH and EM was observed between the MTT microplate and Bactec-MGIT 960TM methods. The observed agreement for RMP was 99% (sensitivity: 90%) and 95.8% for SM (sensitivity 90.9%), lower than those for other drugs. The MTT colourimetric method is an accurate, simple and low-cost alternative in settings with limited resources.
AbstractList We conducted a cross-sectional, hospital-based study between January 2006-March 2008 to estimate the resistance of Mycobacterium tuberculosis to first-line drugs in patients with tuberculosis at a Brazilian hospital. We evaluated the performance of the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] (MTT) microplate assay compared with the Bactec-MGIT 960™ system for mycobacteria testing. The prevalence of resistance in M. tuberculosis was 6.7%. Multidrug-resistance [resistance to rifampicin (RMP) and isoniazid (INH)], INH-resistance and streptomycin (SM)-resistance accounted for 1%, 3.8% and 3.8% of all resistance, respectively, and all isolates were susceptible to ethambutol (EM). The resistance was primary in four cases and acquired in three cases and previous treatment was associated with resistance (p = 0.0129). Among the 119 M. tuberculosis isolates, complete concordance of the results for INH and EM was observed between the MTT microplate and Bactec-MGIT 960TM methods. The observed agreement for RMP was 99% (sensitivity: 90%) and 95.8% for SM (sensitivity 90.9%), lower than those for other drugs. The MTT colourimetric method is an accurate, simple and low-cost alternative in settings with limited resources.
We conducted a cross-sectional, hospital-based study between January 2006-March 2008 to estimate the resistance of Mycobacterium tuberculosis to first-line drugs in patients with tuberculosis at a Brazilian hospital. We evaluated the performance of the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] (MTT) microplate assay compared with the Bactec-MGIT 960 system for mycobacteria testing. The prevalence of resistance in M. tuberculosis was 6.7%. Multidrug-resistance [resistance to rifampicin (RMP) and isoniazid (INH)], INH-resistance and streptomycin (SM)-resistance accounted for 1%, 3.8% and 3.8% of all resistance, respectively, and all isolates were susceptible to ethambutol (EM). The resistance was primary in four cases and acquired in three cases and previous treatment was associated with resistance (p = 0.0129). Among the 119 M. tuberculosis isolates, complete concordance of the results for INH and EM was observed between the MTT microplate and Bactec-MGIT 960 methods. The observed agreement for RMP was 99% (sensitivity: 90%) and 95.8% for SM (sensitivity 90.9%), lower than those for other drugs. The MTT colourimetric method is an accurate, simple and low-cost alternative in settings with limited resources.
We conducted a cross-sectional, hospital-based study between January 2006-March 2008 to estimate the resistance of Mycobacterium tuberculosis to first-line drugs in patients with tuberculosis at a Brazilian hospital. We evaluated the performance of the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] (MTT) microplate assay compared with the Bactec-MGIT 960 system for mycobacteria testing. The prevalence of resistance in M. tuberculosis was 6.7%. Multidrug-resistance [resistance to rifampicin (RMP) and isoniazid (INH)], INH-resistance and streptomycin (SM)-resistance accounted for 1%, 3.8% and 3.8% of all resistance, respectively, and all isolates were susceptible to ethambutol (EM). The resistance was primary in four cases and acquired in three cases and previous treatment was associated with resistance (p = 0.0129). Among the 119 M. tuberculosis isolates, complete concordance of the results for INH and EM was observed between the MTT microplate and Bactec-MGIT 960TM methods. The observed agreement for RMP was 99% (sensitivity: 90%) and 95.8% for SM (sensitivity 90.9%), lower than those for other drugs. The MTT colourimetric method is an accurate, simple and low-cost alternative in settings with limited resources.
Author Ferrazoli, Lucilaine
Resende, Mariângela Ribeiro
Telles, Maria Alice da Silva
Levy, Carlos Emílio
Moretti, Maria Luiza
Villares, Maria Cecília Barison
Ferrari, Michela De Luca
AuthorAffiliation Hospital de Clínicas
Secretaria de Saúde do Estado de São Paulo
Universidade Estadual de Campinas
AuthorAffiliation_xml – name: Hospital de Clínicas
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  givenname: Michela De Luca
  surname: Ferrari
  fullname: Ferrari, Michela De Luca
  organization: Instituto Adolfo Lutz, Secretaria de Saúde do Estado de São Paulo, São Paulo, Brasil
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  givenname: Maria Alice da Silva
  surname: Telles
  fullname: Telles, Maria Alice da Silva
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10.1590/S0074-02762006000500009
10.1590/S0074-02761999000400021
10.1590/S1806-37132004000400007
10.1111/j.1469-0691.2007.01698.x
10.1590/S0037-86822003000100005
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10.1007/PL00015025
10.1586/14737159.6.3.423
10.1086/590010
10.1128/JCM.36.2.362-366.1998
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Issue 5
Keywords tetrazolium
drug susceptibility test
colourimetric method
hospital
resistance
tuberculosis
Language English
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Snippet We conducted a cross-sectional, hospital-based study between January 2006-March 2008 to estimate the resistance of Mycobacterium tuberculosis to first-line...
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SubjectTerms Adult
Anti-Bacterial Agents - pharmacology
Coloring Agents
colourimetric method
Cross-Sectional Studies
drug susceptibility test
Female
hospital
Humans
Male
Microbial Sensitivity Tests - methods
Mycobacterium tuberculosis
Mycobacterium tuberculosis - drug effects
Mycobacterium tuberculosis - isolation & purification
PARASITOLOGY
resistance
Retrospective Studies
tetrazolium
Tetrazolium Salts
Thiazoles
TROPICAL MEDICINE
tuberculosis
Tuberculosis - microbiology
Tuberculosis, Multidrug-Resistant - microbiology
Title Susceptibility of Mycobacterium tuberculosis to first-line antimycobacterial agents in a Brazilian hospital: assessing the utility of the tetrazolium (MTT) microplate assay
URI https://www.ncbi.nlm.nih.gov/pubmed/20835613
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