Alkaline extracellular conditions promote the proliferation and mineralization of a human cementoblast cell line
Aim To investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions. Methodology A human cementoblast cell line was cultured in alkaline media with several pHs (pH 7.6, 8.0 and 8.4) without CO2. Cell numbers, phospho‐p44/42 expression, alkaline phospha...
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Published in | International endodontic journal Vol. 52; no. 5; pp. 639 - 645 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Wiley Subscription Services, Inc
01.05.2019
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Abstract | Aim
To investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions.
Methodology
A human cementoblast cell line was cultured in alkaline media with several pHs (pH 7.6, 8.0 and 8.4) without CO2. Cell numbers, phospho‐p44/42 expression, alkaline phosphatase (ALP) activity and mineralization were evaluated. The significance of differences between groups was assessed using two‐way analysis of variance 15 (ANOVA) followed by Bonferroni's multiple comparison test (α = 0.01).
Results
Cell numbers increased in a time‐dependent manner in the high pH medium groups. Western blot analysis revealed the upregulated expression of phospho‐p44/42 under alkaline conditions. ALP activity was also increased at pH 8.0 and 8.4. Alizarin red staining revealed increased mineralization in the high pH medium groups. The incorporation of the transient receptor potential ankyrin subfamily member 1 (TRPA1) antagonist HC030031 markedly negated the effect on proliferation and mineralization.
Conclusions
Extracellular alkaline conditions promoted the proliferation and mineralization of human cementoblasts in vitro via TRPA1. |
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AbstractList | Aim
To investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions.
Methodology
A human cementoblast cell line was cultured in alkaline media with several pHs (pH 7.6, 8.0 and 8.4) without CO2. Cell numbers, phospho‐p44/42 expression, alkaline phosphatase (ALP) activity and mineralization were evaluated. The significance of differences between groups was assessed using two‐way analysis of variance 15 (ANOVA) followed by Bonferroni's multiple comparison test (α = 0.01).
Results
Cell numbers increased in a time‐dependent manner in the high pH medium groups. Western blot analysis revealed the upregulated expression of phospho‐p44/42 under alkaline conditions. ALP activity was also increased at pH 8.0 and 8.4. Alizarin red staining revealed increased mineralization in the high pH medium groups. The incorporation of the transient receptor potential ankyrin subfamily member 1 (TRPA1) antagonist HC030031 markedly negated the effect on proliferation and mineralization.
Conclusions
Extracellular alkaline conditions promoted the proliferation and mineralization of human cementoblasts in vitro via TRPA1. To investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions. A human cementoblast cell line was cultured in alkaline media with several pHs (pH 7.6, 8.0 and 8.4) without CO . Cell numbers, phospho-p44/42 expression, alkaline phosphatase (ALP) activity and mineralization were evaluated. The significance of differences between groups was assessed using two-way analysis of variance 15 (ANOVA) followed by Bonferroni's multiple comparison test (α = 0.01). Cell numbers increased in a time-dependent manner in the high pH medium groups. Western blot analysis revealed the upregulated expression of phospho-p44/42 under alkaline conditions. ALP activity was also increased at pH 8.0 and 8.4. Alizarin red staining revealed increased mineralization in the high pH medium groups. The incorporation of the transient receptor potential ankyrin subfamily member 1 (TRPA1) antagonist HC030031 markedly negated the effect on proliferation and mineralization. Extracellular alkaline conditions promoted the proliferation and mineralization of human cementoblasts in vitro via TRPA1. Abstract Aim To investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions. Methodology A human cementoblast cell line was cultured in alkaline media with several pH s ( pH 7.6, 8.0 and 8.4) without CO 2 . Cell numbers, phospho‐p44/42 expression, alkaline phosphatase ( ALP ) activity and mineralization were evaluated. The significance of differences between groups was assessed using two‐way analysis of variance 15 ( ANOVA ) followed by Bonferroni's multiple comparison test (α = 0.01). Results Cell numbers increased in a time‐dependent manner in the high pH medium groups. Western blot analysis revealed the upregulated expression of phospho‐p44/42 under alkaline conditions. ALP activity was also increased at pH 8.0 and 8.4. Alizarin red staining revealed increased mineralization in the high pH medium groups. The incorporation of the transient receptor potential ankyrin subfamily member 1 ( TRPA 1) antagonist HC 030031 markedly negated the effect on proliferation and mineralization. Conclusions Extracellular alkaline conditions promoted the proliferation and mineralization of human cementoblasts in vitro via TRPA 1. AIMTo investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions. METHODOLOGYA human cementoblast cell line was cultured in alkaline media with several pHs (pH 7.6, 8.0 and 8.4) without CO2 . Cell numbers, phospho-p44/42 expression, alkaline phosphatase (ALP) activity and mineralization were evaluated. The significance of differences between groups was assessed using two-way analysis of variance 15 (ANOVA) followed by Bonferroni's multiple comparison test (α = 0.01). RESULTSCell numbers increased in a time-dependent manner in the high pH medium groups. Western blot analysis revealed the upregulated expression of phospho-p44/42 under alkaline conditions. ALP activity was also increased at pH 8.0 and 8.4. Alizarin red staining revealed increased mineralization in the high pH medium groups. The incorporation of the transient receptor potential ankyrin subfamily member 1 (TRPA1) antagonist HC030031 markedly negated the effect on proliferation and mineralization. CONCLUSIONSExtracellular alkaline conditions promoted the proliferation and mineralization of human cementoblasts in vitro via TRPA1. |
Author | Ishizuka, H. Kashiwagi, S. Muramatsu, T. Kimura, M. Matsuura, Y. Furusawa, M. Shibukawa, Y. |
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To investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions.
Methodology
A human cementoblast cell line... To investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions. A human cementoblast cell line was cultured in... Abstract Aim To investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions. Methodology A human cementoblast... AimTo investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions.MethodologyA human cementoblast cell line... AIMTo investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions. METHODOLOGYA human cementoblast cell line... |
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SubjectTerms | Alkalies alkaline conditions Alkaline phosphatase Ankyrins calcium Carbon dioxide cementoblast Dentistry Endodontics Mineralization pH effects Proliferation Transient receptor potential proteins TRPA1 Variance analysis |
Title | Alkaline extracellular conditions promote the proliferation and mineralization of a human cementoblast cell line |
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