Median raphe stimulation-induced motor inhibition concurrent with suppression of type 1 and type 2 hippocampal theta

• Published in: Hippocampus Vol. 26; no. 3; pp. 289 - 300
• Main Authors: Bland, Brian H., Bland, Cheryl E., MacIver, M. Bruce
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.03.2016; Wiley Subscription Services, Inc
• Subjects: Analgesics, Opioid - pharmacology; Animals; Atropine - pharmacology; Diphenoxylate - pharmacology; Dorsal Raphe Nucleus - drug effects; Dorsal Raphe Nucleus - physiology; Dose-Response Relationship, Drug; Drug Combinations; Electric Stimulation; electrical stimulation; Exploratory Behavior - drug effects; Exploratory Behavior - physiology; hippocampal; Hippocampus - physiology; Hypothalamus, Posterior - physiology; Male; median raphe; Motor Activity - drug effects; Motor Activity - physiology; motor inhibition; Muscarinic Antagonists - pharmacology; Neural Pathways - physiology; posterior hypothalamus; Rats; Rats, Long-Evans; Running - physiology; suppression; theta; Theta Rhythm - drug effects; Theta Rhythm - physiology; type 1; type 2; Urethane - pharmacology; electrical stimulation; hippocampal; type 2; motor inhibition; type 1; median raphe; suppression; posterior hypothalamus; theta
• ISSN: 1050-9631; 1098-1063
• DOI: 10.1002/hipo.22521

ABSTRACT This study investigated behavioral, anatomical and electrophysiological effects produced by electrical stimulation of posterior hypothalamic (PH) or median raphe (MR) nuclei, independently and during combined stimulation of both PH and MR. These three stimulation conditions were applied during spontaneous behavior in an open field and during PH stimulation‐induced wheel running, while simultaneously recording hippocampal (HPC) field activity. An additional objective was to determine the effects of MR stimulation on Type 1 movement related theta and Type 2 sensory processing related theta. To achieve the latter, when behavioral studies were completed we studied the same rats under urethane anesthesia and then during urethane anesthesia with the addition of atropine sulfate (ATSO4). Here we demonstrated that electrical stimulation of a localized region of the MR nucleus resulted in a profound inhibition of both spontaneously occurring theta related motor behaviors and the theta related motor behaviors induced by electrical stimulation of the PH nucleus. Furthermore, this motor inhibition occurred concurrently with strong suppression of hippocampal theta field oscillations in the freely moving rat, a condition where the theta recorded is Type 2 sensory processing theta occurring coincidently with Type 1 movement related theta (Bland, 1986). Our results indicate that motor inhibition resulted from stimulation of neurons located in the mid central region of the MR, while stimulation in adjacent regions produced variable responses, including movements and theta activity. The present study provided evidence that the pharmacological basis of the suppression of Type 2 sensory processing HPC theta was cholinergic. However, MR inhibition of PH‐induced wheel running was not affected by cholinergic blockade, which blocks Type 2 theta, indicating that MR stimulation‐induced motor inhibition also requires the suppression of Type 1 theta. © 2015 Wiley Periodicals, Inc.