Malonate as a ROS product is associated with pyruvate carboxylase activity in acute myeloid leukaemia cells

• Published in: Cancer & metabolism Vol. 4; no. 1; p. 15
• Main Authors: Reed, Michelle A. C., Ludwig, Christian, Bunce, Christopher M., Khanim, Farhat L., Günther, Ulrich L.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 04.08.2016; BioMed Central
• Subjects: Cancer cells; Care and treatment; Health aspects; Malonates; Pyruvate carboxylase; AML; Malonate; Cancer metabolism; Pyruvate carboxylase

The role of anaplerotic nutrient entry into the Krebs cycle via pyruvate carboxylase has been the subject of increased scrutiny and in particular whether this is dysregulated in cancer. Here, we use a tracer-based NMR analysis involving high-resolution (1)H-(13)C-HSQC spectra to assess site-specific...