Development of flavivirus subviral particles with low cross-reactivity by mutations of a distinct antigenic domain

• Published in: Applied microbiology and biotechnology Vol. 107; no. 24; pp. 7515 - 7529
• Main Authors: Tabata, Koshiro, Itakura, Yukari, Ariizumi, Takuma, Igarashi, Manabu, Kobayashi, Hiroko, Intaruck, Kittiya, Kishimoto, Mai, Kobayashi, Shintaro, Hall, William W., Sasaki, Michihito, Sawa, Hirofumi, Orba, Yasuko
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2023; Springer; Springer Nature B.V
• Subjects: Amino acids; Analysis; Antibodies; Antigen-antibody reactions; Antigenicity; Antigens; Applied Genetics and Molecular Biotechnology; Binding; Biomedical and Life Sciences; Biotechnology; Cross-reactivity; Dengue fever; Encephalitis; Env protein; Enzyme-linked immunosorbent assay; Epitopes; Gene mutations; Immunization; Immunoassays; Immunoglobulin G; Inoculation; Insects; Life Sciences; Microbial Genetics and Genomics; Microbiology; Mutants; Mutation; Vaccination; Vaccines; Vector-borne diseases; Viral envelope proteins; Viruses; West Nile virus; Zika virus; Ireland; Japan; Fusion loop domain; Subviral particle; Serodiagnosis; Mosquito-borne flavivirus; Insect-specific flavivirus; Cross-reactivity

The most conserved fusion loop (FL) domain present in the flavivirus envelope protein has been reported as a dominant epitope for cross-reactive antibodies to mosquito-borne flaviviruses (MBFVs). As a result, establishing accurate serodiagnosis for MBFV infections has been difficult as anti-FL antib...