Start-up and bacterial communities of single-stage nitrogen removal using anammox and partial nitritation (SNAP) for treatment of high strength ammonia wastewater

• Published in: Bioresource technology Vol. 169; pp. 652 - 657
• Main Authors: Zhang, Jianbing, Zhou, Jian, Han, Yi, Zhang, Xiaoguang
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.10.2014; Elsevier
• Subjects: activated sludge; ammonia; Ammonia - isolation & purification; Ammonium Compounds - isolation & purification; Anaerobic ammonium oxidation; Anaerobiosis; Applied sciences; Bacteria - genetics; Bacteria - growth & development; Bacteria - metabolism; bacterial communities; Bacterial community; Base Sequence; Biodegradation, Environmental; biofilm; Biological and medical sciences; Biological treatment of sewage sludges and wastes; Bioreactors; Biotechnology; Completely single-stage autotrophic nitrogen removal; DNA, Ribosomal - genetics; engineering; Environment and pollution; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Methods. Procedures. Technologies; Nitrates - analysis; Nitrification; Nitrites - analysis; nitrogen; Nitrogen - isolation & purification; nitrogen content; Nitrosomonas europaea; Oxidation-Reduction; Phylogeny; Pollution; ribosomal DNA; sequence analysis; Sequence Analysis, DNA; Sequencing batch biofilm reactor (SBBR); Sewage - microbiology; Species Specificity; Surplus dewatered activated sludge; Various methods and equipments; Waste Disposal, Fluid; Waste Water - chemistry; wastewater; Wastewaters; Water Pollutants, Chemical - isolation & purification; Water Purification - methods; Water treatment and pollution; Xanthomonas campestris; Anaerobic ammonium oxidation; Sequencing batch biofilm reactor (SBBR); Completely single-stage autotrophic nitrogen removal; Surplus dewatered activated sludge; Bacterial community; Ammonium; Ammoxidation; Autotrophy; Nitrogen; Nitrosation; Waste water; Ammonia; Anaerobe; Batch process; Biofilm; Bacteria; Oxidation; Reactor; Sequencing; Microbial community; Denitrogenation
• DOI: 10.1016/j.biortech.2014.07.042
• ISSN: 0960-8524; 1873-2976

•The start-up the SNAP process was achieved from dewatered surplus activated sludge.•High rate TN removal around 0.54kgNm−3d−1 was achieved in a single reactor.•16S rDNA amplicon pyrosequencing showed presence of AOB and AnAOB.•Quantitative analysis of dominant bacteria groups arrangement in reactor. In this study, a lab-scale sequencing batch biofilm reactor (SBBR) was used to start up the single-stage nitrogen removal system using anammox and partial nitritation (SNAP) process seeding from surplus activated sludge. The volumetric nitrogen loading rate (vNLR) was firstly 0.075kgNm−3d−1 and then gradually increased to 0.60kgNm−3d−1. A maximal total nitrogen (TN) removal rate of 0.54kgNm−3d−1 was achieved by the SNAP process after 132days operation with NH4+-N and TN removal efficiency of 99.4% and 90.5%, respectively. This reactor may have applications for the SNAP process treating high strength ammonia wastewater. And dewatered surplus activated sludge was recommended as the seed sludge for engineering applications. The dominant bacterial strains were Xanthomonas campestris, Nitrosomonas europaea and Ignavibacterium album, corresponding to the percentage of 24%, 22% and 20%, respectively, based on the 16S rDNA amplicon pyrosequencing of the SNAP sludge.