Squalene epoxidase promotes the chemoresistance of colorectal cancer via (S)-2,3-epoxysqualene-activated NF-κB

• Published in: Cell communication and signaling Vol. 22; no. 1; pp. 278 - 15
• Main Authors: Liu, Qi, Zhang, Yajuan, Li, Huimin, Gao, Hong, Zhou, Yijie, Luo, Dakui, Shan, Zezhi, Yang, Yufei, Weng, Junyong, Li, Qingguo, Yang, Weiwei, Li, Xinxiang
• Format: Journal Article
• Language: English
• Published: England BioMed Central 18.05.2024; BMC
• Subjects: (S)-2,3-epoxysqualene; 5-Fluorouracil; Animals; Antifungal agents; Biosynthesis; Cancer therapies; Cell growth; Cell Line, Tumor; Cell proliferation; Cell Proliferation - drug effects; Cell survival; Chemoresistance; Chemoretherapy; Chemotherapy; Cholesterol; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - drug therapy; Colorectal Neoplasms - genetics; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Drug resistance; Drug Resistance, Neoplasm - drug effects; Drug Resistance, Neoplasm - genetics; Enzymes; Female; Fluorouracil - pharmacology; Fluorouracil - therapeutic use; Humans; Immunoblotting; Immunoprecipitation; Lymphatic system; Male; Medical prognosis; Medical research; Metabolism; Metabolites; Mice; Mice, Inbred BALB C; Mice, Nude; Molecular modelling; NF-kappa B - metabolism; NF-KappaB Inhibitor alpha - genetics; NF-KappaB Inhibitor alpha - metabolism; NF-κB pathway; NF-κB protein; Patients; Sequence analysis; Squalene; Squalene epoxidase; Squalene Monooxygenase - genetics; Squalene Monooxygenase - metabolism; Survival analysis; Terbinafine; Transducin; Tumors; Ubiquitin-protein ligase; Xenograft Model Antitumor Assays; Chemoretherapy; (S)-2,3-epoxysqualene; Squalene epoxidase; Colorectal cancer; NF-κB pathway

While de novo cholesterol biosynthesis plays a crucial role in chemotherapy resistance of colorectal cancer (CRC), the underlying molecular mechanism remains poorly understood. We conducted cell proliferation assays on CRC cells with or without depletion of squalene epoxidase (SQLE), with or without...