Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells

• Published in: Cell Vol. 142; no. 5; pp. 787 - 799
• Main Authors: Kobayashi, Toshihiro, Yamaguchi, Tomoyuki, Hamanaka, Sanae, Kato-Itoh, Megumi, Yamazaki, Yuji, Ibata, Makoto, Sato, Hideyuki, Lee, Youn-Su, Usui, Jo-ichi, Knisely, A.S., Hirabayashi, Masumi, Nakauchi, Hiromitsu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 03.09.2010
• Subjects: Animals; Blastocyst; Body size; Chimera - embryology; DEVBIO; Diabetes Mellitus - chemically induced; Diabetes Mellitus - therapy; Embryonic Development; Gene Knock-In Techniques; Homeodomain Proteins - genetics; Mice; Mice, Inbred Strains; Organogenesis; Pancreas - cytology; Pancreas - embryology; Pluripotent Stem Cells; Rats; Rats, Wistar; STEMCELL; Trans-Activators - genetics; STEMCELL; DEVBIO

The complexity of organogenesis hinders in vitro generation of organs derived from a patient's pluripotent stem cells (PSCs), an ultimate goal of regenerative medicine. Mouse wild-type PSCs injected into Pdx1−/− (pancreatogenesis-disabled) mouse blastocysts developmentally compensated vacancy o...